Rv2795c Resolved · high auto-curated
H37Rv Rv2795c · MTBC0 mtbc0_002974 ·
324 aa · 3126352–3127326 (-) ·
RefSeq NP_217311.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | hypothetical protein |
|---|---|
| MTBC0 PGAP re-annotation | metallophosphoesterase |
| Revised (this work) | Metallophosphoesterase. Pfam: Metallophos (PF00149.34). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
I6YEE1
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | [Acyl-carrier-protein] phosphodiesterase PptH |
| EC (curated) |
EC 3.1.4.14
|
| Curated function | Catalyzes the hydrolysis of the phosphopantetheine group from substrate holo-carrier proteins. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| eggNOG description | Metallophosphoesterase |
| Orthologous group | COG1409 |
| KEGG orthology |
K07498
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.083 · strong purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 31 synonymous, 7 missense, 1 nonsense, 0 frameshift |
| Disruption | 1 distinct premature-stop/frameshift site(s); most common in 0.13% of strains (184) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Metallophos | PF00149.34 | 1.6e-12 | 16–250 | Calcineurin-like phosphoesterase |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: pptT (4'-phosphopantetheinyl transferase), high confidence from genomic context alone (score 946 excluding text-mining). This association is the citable seed of a function hypothesis for this hypothetical protein.
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2794c pptT |
4'-phosphopantetheinyl transferase | 992 | 946 ctx | neighborhood:882 textmining:870 |
Rv2793c truB |
tRNA pseudouridine synthase B | 887 | 887 ctx | neighborhood:882 |
Rv2790c ltp1 |
lipid-transfer protein | 719 | 719 ctx | neighborhood:712 |
Rv2789c fadE21 |
acyl-CoA dehydrogenase FadE21 | 704 | 704 ctx | neighborhood:697 |
Rv2799 |
membrane protein | 691 | 691 ctx | neighborhood:691 |
Rv2798c hyp |
hypothetical protein | 546 | 546 ctx | neighborhood:542 |
Rv2797c hyp |
hypothetical protein | 545 | 545 ctx | neighborhood:542 |
Rv2796c lppV |
lipoprotein LppV | 544 | 543 ctx | neighborhood:542 |
Rv2792c |
resolvase | 481 | 481 ctx | neighborhood:474 |
Rv2791c tnpB |
transposase | 480 | 480 ctx | neighborhood:474 |
Rv2800 |
hydrolase | 475 | 456 ctx | neighborhood:456 |
Rv1663 pks17 |
polyketide synthase | 453 | 433 ctx | cooccurence:415 |
Rv0359 rip2 |
zinc metalloprotease | 422 | 422 ctx | cooccurence:403 |
Rv0199 |
membrane protein | 439 | 415 | experimental:403 |
Rv0200 |
transmembrane protein | 438 | 413 | experimental:403 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: hypothetical protein
- MTBC0 PGAP product: metallophosphoesterase
- Pfam (hmmscan --cut_ga): Metallophos PF00149.34 (E=2e-12)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_217311.1)
- Domains: Pfam-A via hmmscan --cut_ga — Metallophos (PF00149.34)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1409 - Curated reference: UniProt I6YEE1 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
24 functional partner(s); context anchor
pptT - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_002974|Rv2795c| MTWKGSGQETVGAEPTLWAISDLHTGHLGNKPVAESLYPSSPDDWLIVAGDVAERTDEIRWSLDLLRRRFAKVIWVPGNHELWTTNRDPMQIFGRARYDYLVNMCDEMGVVTPEHPFPVWTERGGPATIVPMFLLYDYSFLPEGANSKAEGVAIAKERNVVATDEFLLSPEPYPTRDAWCHERVAATRARLEQLDWMQPTVLVNHFPLLRQPCDALFYPEFSLWCGTTKTADWHTRYNAVCSVYGHLHIPRTTWYDGVRFEEVSVGYPREWRRRKPYSWLRQVLPDPQYAPGYLNDFGGHFVITPEMRTQAAQFRERLRQRQSR