Rv2797c Family assigned · medium auto-curated
H37Rv Rv2797c · MTBC0 mtbc0_002976 ·
562 aa · 3128034–3129722 (-) ·
RefSeq NP_217313.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | hypothetical protein |
|---|---|
| MTBC0 PGAP re-annotation | alpha/beta hydrolase family protein |
| Revised (this work) | Alpha/beta hydrolase family protein. Pfam: Abhydrolase_8 (PF06259.19). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P71654
TrEMBL · unreviewed
· Predicted
|
|---|---|
| UniProt name | DUF1023 domain-containing protein |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
E Amino acid transport and metabolism
|
|---|---|
| eggNOG description | Alpha/beta hydrolase |
| Orthologous group | COG1506 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.218 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 25 synonymous, 14 missense, 1 nonsense, 0 frameshift |
| Disruption | 1 distinct premature-stop/frameshift site(s); most common in 0.46% of strains (664) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Abhydrolase_8 | PF06259.19 | 6.4e-74 | 277–465 | Alpha/beta hydrolase |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: lppV (lipoprotein LppV), high confidence from genomic context alone (score 886 excluding text-mining). This association is the citable seed of a function hypothesis for this hypothetical protein.
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2796c lppV |
lipoprotein LppV | 886 | 886 ctx | neighborhood:882 |
Rv2798c hyp |
hypothetical protein | 883 | 883 ctx | neighborhood:882 |
Rv2490c PE_PGRS43 |
PE-PGRS family protein PE_PGRS43 | 751 | 751 ctx | cooccurence:751 |
Rv3879c espK |
ESX-1 secretion-associated protein EspK | 743 | 743 ctx | cooccurence:743 |
Rv0341 iniB |
isoniazid inducible protein IniB | 741 | 741 ctx | cooccurence:739 |
Rv3347c PPE55 |
PPE family protein PPE55 | 740 | 741 ctx | cooccurence:739 |
Rv3350c PPE56 |
PPE family protein PPE56 | 737 | 738 ctx | cooccurence:736 |
Rv1004c |
membrane protein | 735 | 735 ctx | cooccurence:734 |
Rv0355c PPE8 |
PPE family protein PPE8 | 733 | 734 ctx | cooccurence:732 |
Rv3343c PPE54 |
PPE family protein PPE54 | 727 | 728 ctx | cooccurence:726 |
Rv2098c PE_PGRS36 |
PE-PGRS family protein PE_PGRS36; Rv2098c, (MTCY49.38c), len: 434 aa. PE_PGRS36,Member of the Mycobacterium tuberculosis PE family, PGRS sub | 727 | 728 ctx | cooccurence:725 |
Rv1651c PE_PGRS30 |
PE-PGRS family protein PE_PGRS30 | 725 | 725 ctx | cooccurence:725 |
Rv1917c PPE34 |
PPE family protein PPE34 | 719 | 720 ctx | cooccurence:718 |
Rv1452c PE_PGRS28 |
PE-PGRS family protein PE_PGRS28 | 716 | 716 ctx | cooccurence:716 |
Rv2209 |
integral membrane protein | 713 | 713 ctx | cooccurence:712 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: hypothetical protein
- MTBC0 PGAP product: alpha/beta hydrolase family protein
- Pfam (hmmscan --cut_ga): Abhydrolase_8 PF06259.19 (E=6e-74)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_217313.1)
- Domains: Pfam-A via hmmscan --cut_ga — Abhydrolase_8 (PF06259.19)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1506 - Curated reference: UniProt P71654 (TrEMBL, unreviewed; Predicted)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
82 functional partner(s); context anchor
lppV - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_002976|Rv2797c| MPLTVADIDRWNAQAVREVFHAASARAEVTFEASRQLAALSIFANSGGKTAEAAAHHNAGIRRDLDAHGNEALAVARAADRAADGIVKVQSELAALRHAAAAAELTIDALINRVVPIPGLRSTEAQWARTLAKQTELQAELDAIMAEANAVDEELASAVNMADGDAPIPADSGPPVGPEGLTPTQLASDANEERLREERARLQAHLERLQAEYDQLSVRAARDYHNGILDGDAVGRLAALTDELSAARGRLGELDAVDEALSRAPETYLTQLQIPEDPNQQVLAAVAVGNPDTAANVSVTVPGVGSTTRGALPGMVTEARDLRSEVIRQLNAAGKPASVATIAWMGYHPPPNPLDTGSAGDLWQTMTDGQAHAGAADLSRYLQQVRANNPSGHLTVLGHSYGSLTASLALQDLDAQSAHPVNDVVFYGSPGLELYSPAQLGLDHGHAYVMQAPHDLITNLVAPLAPLHGWGLDPYLTPGFTELSSQAGFDPGGIWRDGVYAHGDYPRSFLDAAGQPQLRMSGYNLAAIAAGLPDNTVGPPLLPPILGGGMPAAPGPALRGGR