Rv2787 Family assigned · medium auto-curated
H37Rv Rv2787 · MTBC0 mtbc0_002966 ·
587 aa · 3117526–3119289 (+) ·
RefSeq NP_217303.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | hypothetical protein |
|---|---|
| MTBC0 PGAP re-annotation | AAA family ATPase |
| Revised (this work) | AAA family ATPase. Pfam: T7SS_EccA1_N (PF21545.4), CbiA (PF01656.30). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
O33329
TrEMBL · unreviewed
· Predicted
|
|---|---|
| UniProt name | Conserved hypothetical alanine rich protein |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
D Cell cycle control, cell division, chromosome partitioning
|
|---|---|
| eggNOG description | AAA domain |
| Orthologous group | COG0455 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.37 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 9 synonymous, 10 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
T7SS_EccA1_N | PF21545.4 | 9.4e-40 | 17–251 | T7SS, ESX-1 secretion system protein EccA1, N-terminal domain |
CbiA | PF01656.30 | 2.0e-08 | 323–468 | CobQ/CobB/MinD/ParA nucleotide binding domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: sirR (transcriptional repressor SirR), medium confidence from genomic context alone (score 567 excluding text-mining). This association is the citable seed of a function hypothesis for this hypothetical protein.
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2788 sirR |
transcriptional repressor SirR | 566 | 567 ctx | neighborhood:567 |
Rv2209 |
integral membrane protein | 502 | 502 ctx | cooccurence:502 |
Rv1651c PE_PGRS30 |
PE-PGRS family protein PE_PGRS30 | 500 | 501 ctx | cooccurence:490 |
Rv1452c PE_PGRS28 |
PE-PGRS family protein PE_PGRS28 | 494 | 495 ctx | cooccurence:484 |
Rv0355c PPE8 |
PPE family protein PPE8 | 491 | 491 ctx | cooccurence:488 |
Rv2786c ribF |
bifunctional riboflavin kinase /FMN adenylyltransferase | 488 | 488 ctx | neighborhood:488 |
Rv1366 hyp |
hypothetical protein | 482 | 482 ctx | cooccurence:454 |
Rv2819c csm5 |
CRISPR type III-associated RAMP protein Csm5 | 480 | 481 ctx | cooccurence:473 |
Rv3350c PPE56 |
PPE family protein PPE56 | 477 | 477 ctx | cooccurence:473 |
Rv1004c |
membrane protein | 498 | 473 ctx | cooccurence:472 |
Rv3347c PPE55 |
PPE family protein PPE55 | 470 | 470 ctx | cooccurence:468 |
Rv2490c PE_PGRS43 |
PE-PGRS family protein PE_PGRS43 | 457 | 458 ctx | cooccurence:446 |
Rv3286c sigF |
RNA polymerase sigma factor SigF | 471 | 438 | coexpression:419 |
Rv2308 hyp |
hypothetical protein | 436 | 437 ctx | cooccurence:432 |
Rv3343c PPE54 |
PPE family protein PPE54 | 434 | 435 ctx | cooccurence:431 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: hypothetical protein
- MTBC0 PGAP product: AAA family ATPase
- Pfam (hmmscan --cut_ga): T7SS_EccA1_N PF21545.4 (E=9e-40), CbiA PF01656.30 (E=2e-08)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_217303.1)
- Domains: Pfam-A via hmmscan --cut_ga — T7SS_EccA1_N (PF21545.4), CbiA (PF01656.30)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0455 - Curated reference: UniProt O33329 (TrEMBL, unreviewed; Predicted)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
23 functional partner(s); context anchor
sirR - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_002966|Rv2787| MSTFRECRSMFDAAVKSYQSGDLANARAAFGRLTVENPDMSDGWLGLLACGDHHLDTLAGAHQHSEALYSETRRVGLTDGELSAVVMAPMYLGLRVWSRATIGLAYASALIIADRHDEAAATLDDPVITEDTGAAQYRQFVMATLFHKTRSWSNLLKVTEISPPSGATDVRDEVADAVAALASTAAASLGQFQFALELAEQVSTTNPRVTADVTLTRAWCLRELGDDDAARVALSATTTGDAPRTNTTAEQAGSPQPKFRHPYDDGRDLLVARRRPPAGDGWRKAVTKMTFGRVNPEPSAKREQTDELIQRICAPLADVHKLAFVSAKGGVGKTTMTVLVGNAVARLRGDRVMAVDVDADLGDLSARFSERGGPQTNIEHFVSSQHTKRYADVRVHTVMNKDRLEMLGAQNDPRSTYKFGPEDYGAAMQILETHCNVILLDCGTPVNGPLFSNILNDVTGLVVVASEDVRGVEGALVTLDWLGAHGFGRLLQHTVVVLNAIQKTRSLVDCGAAENQFRKRVPDFFRIPYDPHLATGLAVDFSSLKRRTRNAVLDLAGGLAQHYPASRVRPRGEDSWKTWIETMRQVG