mrr Resolved · high auto-curated
H37Rv Rv2528c · MTBC0 mtbc0_002692 ·
306 aa · 2874641–2875561 (-) ·
RefSeq NP_217044.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | restriction system protein |
|---|---|
| MTBC0 PGAP re-annotation | restriction endonuclease |
| Revised (this work) | Restriction endonuclease. Pfam: Mrr_N (PF14338.13), Mrr_cat (PF04471.19), NA-iREase1 (PF22722.3), Mrr_cat_2 (PF13156.13). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
I6Y9K2
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Probable restriction system protein Mrr |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
L Replication, recombination and repair
|
|---|---|
| Preferred name | mrr |
| eggNOG description | Restriction endonuclease |
| Orthologous group | COG1715 |
| KEGG orthology |
K07448
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.339 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 8 synonymous, 8 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Mrr_N | PF14338.13 | 5.2e-29 | 7–91 | Mrr N-terminal domain |
Mrr_cat | PF04471.19 | 1.0e-37 | 162–280 | Restriction endonuclease |
NA-iREase1 | PF22722.3 | 1.2e-10 | 169–277 | NACHT-associated inactive Restriction Endonuclease 1 |
Mrr_cat_2 | PF13156.13 | 2.9e-06 | 190–283 | Restriction endonuclease |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: vapC38 (ribonuclease VapC38), medium confidence from genomic context alone (score 410 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2756c hsdM |
type I restriction/modification system DNA methylase HsdM | 643 | 589 | coexpression:423 |
Rv2529 hyp |
hypothetical protein | 776 | 498 ctx | neighborhood:491 textmining:572 |
Rv2494 vapC38 |
ribonuclease VapC38 | 410 | 410 ctx | cooccurence:410 |
Rv2755c hsdS.1 |
Rv2755c, (MTV002.20c), len: 91 aa. Possible hsdS.1,fragment of type I restriction/modification system specificity determinant (S protein), s | 732 | 367 | textmining:594 |
Rv2761c hsdS |
type I restriction/modification system specificity determinant HsdS | 718 | 184 | textmining:669 |
Rv1048c hyp |
hypothetical protein | 575 | 100 | textmining:547 |
Rv2527 vapC17 |
ribonuclease VapC17 | 443 | 61 | textmining:432 |
Rv0620 galK |
galactokinase | 892 | 52 | textmining:892 |
Rv0325 hyp |
hypothetical protein | 417 | 52 | textmining:411 |
Rv1833c dhmA2 |
haloalkane dehalogenase | 544 | 46 | textmining:542 |
Rv0326 hyp |
hypothetical protein | 514 | 46 | textmining:512 |
Rv1531 hyp |
hypothetical protein | 521 | 44 | textmining:520 |
Rv2533c nusB |
N utilization substance protein B | 437 | 44 | textmining:436 |
Rv0682 rpsL |
30S ribosomal protein S12 | 574 | 42 | textmining:574 |
Rv1767 hyp |
hypothetical protein | 542 | 42 | textmining:542 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: restriction system protein
- MTBC0 PGAP product: restriction endonuclease
- Pfam (hmmscan --cut_ga): Mrr_N PF14338.13 (E=5e-29), Mrr_cat PF04471.19 (E=1e-37), NA-iREase1 PF22722.3 (E=1e-10), Mrr_cat_2 PF13156.13 (E=3e-06)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_217044.1)
- Domains: Pfam-A via hmmscan --cut_ga — Mrr_N (PF14338.13), Mrr_cat (PF04471.19), NA-iREase1 (PF22722.3), Mrr_cat_2 (PF13156.13)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1715 - Curated reference: UniProt I6Y9K2 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
23 functional partner(s); context anchor
vapC38 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_002692|Rv2528c|mrr MTIPDAQTLMRPILAYLADGQAKSAKDVIAAMSDEFGLSDDERAQMLPSGRQRTMYDRVHWSLTHMSQAGLLDRPTRGHVQVTDTGRQVLKAHPERVDMAVLREFPSYIAFRERTKAKQPVDATAKRPSGDDVQVSPEDLIDAALAENRAAVEGEILKKALTLSPTGFEDLVIRLLEAMGYGRAGAVERTSASGDAGIDGIISQDPLGLDRIYVQAKRYAVDQTIGRPKIHEFAGALLGKQGDRGVYITTSSFSRGAREEAERINARIELIDGARLAELLVRYRVGVQAVQTVELLRLDEDFFDGL