Rv2366c Family assigned · medium auto-curated
H37Rv Rv2366c · MTBC0 mtbc0_002518 ·
435 aa · 2671253–2672560 (-) ·
RefSeq NP_216882.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | transmembrane protein |
|---|---|
| MTBC0 PGAP re-annotation | hemolysin family protein |
| Revised (this work) | Hemolysin family protein. Pfam: CNNM (PF01595.26), CBS (PF00571.34), CorC_HlyC (PF03471.23). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WFP1
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | UPF0053 protein Rv2366c |
UniProt still lists this protein as UPF0053 protein Rv2366c; the revised annotation above is ahead of the current UniProt record.
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| Preferred name | corC |
| eggNOG description | CBS domain |
| Orthologous group | COG1253 |
| Gene Ontology (6) |
GO:0005575, GO:0005623, GO:0005886, GO:0016020, GO:0044464, GO:0071944
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 1.78 · diversifying/relaxed |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 1 synonymous, 5 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
CNNM | PF01595.26 | 5.2e-35 | 9–185 | Cyclin M transmembrane N-terminal domain |
CBS | PF00571.34 | 5.1e-07 | 269–323 | CBS domain |
CorC_HlyC | PF03471.23 | 7.6e-12 | 341–400 | Transporter associated domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: ybeY (endoribonuclease), high confidence from genomic context alone (score 988 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2367c ybeY |
endoribonuclease | 988 | 988 ctx | neighborhood:882 coexpression:887 |
Rv2365c hyp |
hypothetical protein | 982 | 983 ctx | neighborhood:882 coexpression:860 |
Rv2368c phoH1 |
phosphate starvation-inducible protein PhoH | 976 | 976 ctx | neighborhood:881 coexpression:806 |
Rv2364c era |
GTPase Era | 966 | 966 ctx | neighborhood:791 coexpression:844 |
Rv3608c folP1 |
dihydropteroate synthase | 793 | 793 | coexpression:793 |
Rv2369c hyp |
hypothetical protein | 775 | 774 ctx | neighborhood:773 |
Rv2370c hyp |
hypothetical protein | 774 | 773 ctx | neighborhood:773 |
Rv2373c dnaJ2 |
chaperone protein DnaJ | 689 | 690 ctx | neighborhood:544 |
Rv2372c rsmE |
rRNA small subunit methyltransferase E | 548 | 548 ctx | neighborhood:544 |
Rv2374c hrcA |
heat-inducible transcription repressor HrcA | 535 | 536 ctx | neighborhood:529 |
Rv2371 PE_PGRS40 |
PE-PGRS family protein PE_PGRS40 | 497 | 497 ctx | neighborhood:497 |
Rv3455c truA |
tRNA pseudouridine synthase A | 454 | 455 ctx | cooccurence:428 |
Rv2553c mltG |
membrane protein | 436 | 433 | coexpression:415 |
Rv2725c hflX |
GTP-binding protein HflX | 401 | 402 | |
Rv0352 dnaJ1 |
chaperone protein DnaJ | 401 | 401 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: transmembrane protein
- MTBC0 PGAP product: hemolysin family protein
- Pfam (hmmscan --cut_ga): CNNM PF01595.26 (E=5e-35), CBS PF00571.34 (E=5e-07), CorC_HlyC PF03471.23 (E=8e-12)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216882.1)
- Domains: Pfam-A via hmmscan --cut_ga — CNNM (PF01595.26), CBS (PF00571.34), CorC_HlyC (PF03471.23)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1253 - Curated reference: UniProt P9WFP1 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
16 functional partner(s); context anchor
ybeY - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_002518|Rv2366c| MTGYYQLLGSIVLIGLGGLFAAIDAAISTVSPARVDELVRDQRPGAGSLRKVMADRPRYVNLVVLLRTSCEITATALLVVFIRYHFSMVWGLYLAAGIMVLASFVVVGVGPRTLGRQNAYSISLATALPLRLISWLLMPISRLLVLLGNALTPGRGFRNGPFASEIELREVVDLAQQRGVVAADERRMIESVFELGDTPAREVMVPRTEMIWIESDKTAGQAMTLAVRSGHSRIPVIGENVDDIVGVVYLKDLVEQTFCSTNGGRETTVARVMRPAVFVPDSKPLDALLREMQRDRNHMALLVDEYGAIAGLVSIEDVLEEIVGEIADEYDQAETAPVEDLGDKRFRVSARLPIEDVGELYGVEFDDDLDVDTVGGLLALELGRVPLPGAEVISHGLRLHAEGGTDHRGRVRIGTVLLSPAEPDGADDEEADHPG