Rv2184c Family assigned · medium auto-curated
H37Rv Rv2184c · MTBC0 - ·
379 aa · 2445807–2446946 (-) ·
RefSeq NP_216700.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | hypothetical protein |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | Contains ArsA_ATPase (PF02374.22), ArsA_HSP20 (PF17886.8) domain(s); putative function inferred from the domain architecture. |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
O53518
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Protein Rv2184c |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
P Inorganic ion transport and metabolism
|
|---|---|
| Preferred name | arsA |
| eggNOG description | anion-transporting ATPase |
| Orthologous group | COG0003 |
| EC number |
EC 3.6.3.16
|
| KEGG orthology |
K01551
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.076 · strong purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 10 synonymous, 2 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
ArsA_ATPase | PF02374.22 | 2.9e-25 | 1–276 | Anion-transporting ATPase |
ArsA_HSP20 | PF17886.8 | 2.1e-14 | 310–370 | HSP20-like domain found in ArsA |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv2182c (1-acylglycerol-3-phosphate O-acyltransferase), high confidence from genomic context alone (score 819 excluding text-mining). This association is the citable seed of a function hypothesis for this hypothetical protein.
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2183c hyp |
hypothetical protein | 897 | 897 ctx | neighborhood:882 |
Rv2182c |
1-acylglycerol-3-phosphate O-acyltransferase | 819 | 819 ctx | neighborhood:782 |
Rv2185c TB16.3 hyp |
hypothetical protein | 765 | 766 ctx | neighborhood:763 |
Rv2187 fadD15 |
long-chain-fatty-acid--CoA ligase FadD15 | 670 | 670 ctx | neighborhood:649 |
Rv1691 hyp |
hypothetical protein | 575 | 554 | database:410 |
Rv3529c hyp |
hypothetical protein | 575 | 554 | database:410 |
Rv2267c stf3 hyp |
hypothetical protein | 575 | 554 | database:410 |
Rv2138 lppL |
lipoprotein LppL | 522 | 522 ctx | cooccurence:522 |
Rv2186c hyp |
hypothetical protein | 473 | 473 ctx | neighborhood:473 |
Rv1486c hyp |
hypothetical protein | 471 | 471 ctx | cooccurence:468 |
Rv1385 pyrF |
orotidine 5'-phosphate decarboxylase | 468 | 447 | experimental:440 |
Rv0817c lmeA hyp |
hypothetical protein | 427 | 427 ctx | cooccurence:427 |
Rv1334 mec |
[CysO | 412 | 413 | |
Rv1371 |
membrane protein | 440 | 405 | |
Rv0435c |
ATPase | 406 | 376 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): hypothetical protein
- Pfam (hmmscan --cut_ga): ArsA_ATPase PF02374.22 (E=3e-25), ArsA_HSP20 PF17886.8 (E=2e-14)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216700.1)
- Domains: Pfam-A via hmmscan --cut_ga — ArsA_ATPase (PF02374.22), ArsA_HSP20 (PF17886.8)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0003 - Curated reference: UniProt O53518 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
16 functional partner(s); context anchor
Rv2182c - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv2184c| MVVSTDQAHSLGDVLGIAVPPTGQGDPVRVLAYDPEAGGGFLDALALDTLALLEGRWLHVVETLDRRFPGSELSSIAPEELCALPGIQEVLGLHAVGELAAARRWDRIVVDCASTADALRMLTLPATFGLYVERAWPRHRRLSIGADDGRSAVLAELLERIRASVERLSTLLTDGALVSAHLVLTPERVVAAEAVRTLGSLALMGVRVEELLVNQLLVQDENYEYRSLPDHPAFHWYAERIGEQRAVLDDLDATIGDVALVLVPHLAGEPIGPKALGGLLDSARRRQGSAPPGPLQPIVDLESGSGLASIYRLRLALPQLDPGTLTLGRADDDLIVSAGGMRRRVRLASVLRRCTVLDAHLRGGELTVRFRPNPEVWPT