Rv2951c Resolved · high auto-curated
H37Rv Rv2951c · MTBC0 mtbc0_003133 ·
381 aa · 3324278–3325423 (-) ·
RefSeq NP_217467.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | phthiodiolone/phenolphthiodiolone dimycocerosates ketoreductase |
|---|---|
| MTBC0 PGAP re-annotation | phthiodiolone/phenolphthiodiolone dimycocerosates ketoreductase |
| Revised (this work) | Phthiodiolone/phenolphthiodiolone dimycocerosates ketoreductase. Pfam: Bac_luciferase (PF00296.27). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WIB7
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Phthiodiolone/phenolphthiodiolone dimycocerosates ketoreductase |
| EC (curated) |
EC 1.2.-.-
|
| Curated function | Catalyzes the reduction of the keto moiety of phthiodiolone dimycocerosates (DIM B) and glycosylated phenolphthiodiolone dimycocerosates to form the intermediate compounds phthiotriol and glycosylated phenolphthiotriol dimycocerosates during phthiocerol dimycocerosates (DIM A) and glycosylated phenolphthiocerol dimycocerosates (PGL) biosynthesis. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
C Energy production and conversion
|
|---|---|
| eggNOG description | Catalyzes the reduction of the keto moiety of phthiodiolone dimycocerosates (DIM B) and glycosylated phenolphthiodiolone dimycocerosates to form the intermediate compounds phthiotriol and glycosylated phenolphthiotriol dimycocerosates during phthiocerol dimycocerosates (DIM A) and glycosylated phenolphthiocerol dimycocerosates (PGL) biosynthesis |
| Orthologous group | COG2141 |
| KEGG orthology |
K14728
|
| Gene Ontology (11) |
GO:0005575, GO:0005622, GO:0005623, GO:0005737, GO:0005829, GO:0005886, GO:0016020, GO:0044424, GO:0044444, GO:0044464, GO:0071944
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | n/a |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 0 synonymous, 3 missense, 1 nonsense, 0 frameshift |
| Disruption | 1 distinct premature-stop/frameshift site(s); most common in 0.17% of strains (244) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Bac_luciferase | PF00296.27 | 4.4e-49 | 30–346 | Luciferase-like monooxygenase |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv2952 (phthiotriol/phenolphthiotriol dimycocerosates methyltransferase), high confidence from genomic context alone (score 799 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2952 |
phthiotriol/phenolphthiotriol dimycocerosates methyltransferase | 881 | 799 ctx | neighborhood:597 database:500 textmining:435 |
Rv3261 fbiA |
2-phospho-L-lactate transferase | 760 | 747 ctx | cooccurence:737 |
Rv3093c |
oxidoreductase | 742 | 743 ctx | cooccurence:742 |
Rv3262 fbiB |
coenzyme F420:L-glutamate ligase | 765 | 740 ctx | cooccurence:706 |
Rv2893 |
oxidoreductase | 732 | 732 ctx | cooccurence:732 |
Rv2953 |
trans-acting enoyl reductase | 963 | 730 ctx | neighborhood:469 database:500 textmining:870 |
Rv3178 |
nitroreductase | 740 | 724 ctx | cooccurence:724 |
Rv2161c hyp |
hypothetical protein | 718 | 718 ctx | cooccurence:717 |
Rv3547 ddn |
deazaflavin-dependent nitroreductase | 720 | 703 ctx | cooccurence:702 |
Rv3072c hyp |
hypothetical protein | 706 | 691 ctx | cooccurence:690 |
Rv3079c hyp |
hypothetical protein | 679 | 679 ctx | cooccurence:679 |
Rv2983 cofC |
2-phospho-L-lactate guanylyltransferase | 699 | 668 ctx | cooccurence:657 |
Rv1558 hyp |
hypothetical protein | 744 | 665 ctx | cooccurence:662 |
Rv1261c hyp |
hypothetical protein | 631 | 632 ctx | cooccurence:628 |
Rv0940c |
oxidoreductase | 635 | 616 ctx | cooccurence:615 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: phthiodiolone/phenolphthiodiolone dimycocerosates ketoreductase
- MTBC0 PGAP product: phthiodiolone/phenolphthiodiolone dimycocerosates ketoreductase
- Pfam (hmmscan --cut_ga): Bac_luciferase PF00296.27 (E=4e-49)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_217467.1)
- Domains: Pfam-A via hmmscan --cut_ga — Bac_luciferase (PF00296.27)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG2141 - Curated reference: UniProt P9WIB7 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
26 functional partner(s); context anchor
Rv2952 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_003133|Rv2951c| MGGLRFGFVDALVHSRLPPTLPARSSMAAATVMGADSYWVGDHLNALVPRSIATSEYLGIAAKFVPKIDANYEPWTMLGNLAFGLPSRLRLGVCVTDAGRRNPAVTAQAAATLHLLTRGRAILGIGVGEREGNEPYGVEWTKPVARFEEALATIRALWNSNGELISRESPYFPLHNALFDLPPYRGKWPEIWVAAHGPRMLRATGRYADAWIPIVVVRPSDYSRALEAVRSAASDAGRDPMSITPAAVRGIITGRNRDDVEEALESVVVKMTALGVPGEAWARHGVEHPMGADFSGVQDIIPQTMDKQTVLSYAAKVPAALMKEVVFSGTPDEVIDQVAEWRDHGLRYVVLINGSLVNPSLRKTVTAVLPHAKVLRGLKKL