cyp132 Resolved · high auto-curated
H37Rv Rv1394c · MTBC0 - ·
461 aa · 1569584–1570969 (-) ·
RefSeq YP_177807.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | cytochrome P450 Cyp132 |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | Cytochrome P450 Cyp132. Pfam: p450 (PF00067.28). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
P9WPN3
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Putative cytochrome P450 132 |
| EC (curated) |
EC 1.14.-.-
|
UniProt still lists this protein as Putative cytochrome P450 132; the revised annotation above is ahead of the current UniProt record.
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
C Energy production and conversion
|
|---|---|
| Preferred name | cyp132 |
| eggNOG description | Cytochrome P450 |
| Orthologous group | COG2124 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.333 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 8 synonymous, 7 missense, 1 nonsense, 0 frameshift |
| Disruption | 1 distinct premature-stop/frameshift site(s); most common in 0.20% of strains (285) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
p450 | PF00067.28 | 1.2e-64 | 45–443 | Cytochrome P450 |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv1393c (monoxygenase), high confidence from genomic context alone (score 985 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1393c |
monoxygenase | 985 | 985 ctx | neighborhood:882 coexpression:860 |
Rv3800c pks13 |
polyketide synthase | 943 | 938 | experimental:891 |
Rv2380c mbtE |
peptide synthetase | 809 | 800 | experimental:689 |
Rv1937 |
oxygenase | 812 | 785 | experimental:478 |
Rv1395 |
HTH-type transcriptional regulator | 862 | 767 ctx | neighborhood:764 textmining:433 |
Rv3554 fdxB |
electron transfer protein FdxB | 742 | 710 | |
Rv2268c cyp128 |
cytochrome P450 Cyp128 | 860 | 703 ctx | cooccurence:700 textmining:548 |
Rv2932 ppsB |
phthiocerol synthesis polyketide synthase type I PpsB | 719 | 701 | experimental:460 |
Rv0766c cyp123 |
cytochrome P450 Cyp123 | 795 | 689 ctx | cooccurence:685 |
Rv0719 rplF |
50S ribosomal protein L6 | 689 | 689 | experimental:412 database:493 |
Rv1629 polA |
DNA polymerase I | 706 | 688 | database:638 |
Rv3571 kshB |
3-ketosteroid-9-alpha-hydroxylase reductase subunit | 703 | 686 | |
Rv2946c pks1 |
polyketide synthase | 716 | 682 | experimental:460 |
Rv3825c pks2 |
phthioceranic/hydroxyphthioceranic acid synthase | 699 | 666 | |
Rv1785c cyp143 |
cytochrome P450 Cyp143 | 831 | 664 ctx | cooccurence:662 textmining:518 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): cytochrome P450 Cyp132
- Pfam (hmmscan --cut_ga): p450 PF00067.28 (E=1e-64)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq YP_177807.1)
- Domains: Pfam-A via hmmscan --cut_ga — p450 (PF00067.28)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG2124 - Curated reference: UniProt P9WPN3 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
123 functional partner(s); context anchor
Rv1393c - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv1394c|cyp132 MATATTQRPLKGPAKRMSTWTMTREAITIGFDAGDGFLGRLRGSDITRFRCAGRRFVSISHPDYVDHVLHEARLKYVKSDEYGPIRATAGLNLLTDEGDSWARHRGALNSTFARRHLRGLVGLMIDPIADVTAARVPGAQFDMHQSMVETTLRVVANALFSQDFGPLVQSMHDLATRGLRRAEKLERLGLWGLMPRTVYDTLIWCIYSGVHLPPPLREMQEITLTLDRAINSVIDRRLAEPTNSADLLNVLLSADGGIWPRQRVRDEALTFMLAGHETTANAMSWFWYLMALNPQARDHMLTELDDVLGMRRPTADDLGKLAWTTACLQESQRYFSSVWIIAREAVDDDIIDGHRIRRGTTVVIPIHHIHHDPRWWPDPDRFDPGRFLRCPTDRPRCAYLPFGGGRRICIGQSFALMEMVLMAAIMSQHFTFDLAPGYHVELEATLTLRPKHGVHVIGRRR