PE_PGRS25 Family assigned · medium auto-curated
H37Rv Rv1396c · MTBC0 - ·
576 aa · 1572127–1573857 (-) ·
RefSeq YP_177809.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | PE-PGRS family protein PE_PGRS25 |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | PE-PGRS family protein PE_PGRS25. Pfam: PE (PF00934.26), PGRS (PF21526.3). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
P71664
TrEMBL · unreviewed
· Predicted
|
|---|---|
| UniProt name | PE-PGRS family protein PE_PGRS25 |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
I Lipid transport and metabolism
|
|---|---|
| eggNOG description | acetylesterase activity |
| Orthologous group | COG0657 |
| EC number |
EC 3.1.1.3
|
| KEGG orthology |
K01046
|
| KEGG pathways |
map00561, map01100
|
| KEGG modules |
M00098
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 1.019 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 4 synonymous, 9 missense, 1 nonsense, 3 frameshift |
| Disruption | 4 distinct premature-stop/frameshift site(s); most common in 4.74% of strains (6890) · convergent |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
PE | PF00934.26 | 3.7e-34 | 4–94 | PE family |
PGRS | PF21526.3 | 5.3e-13 | 116–185 | PGRS repeats |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: vapC10 (ribonuclease VapC10), medium confidence from genomic context alone (score 452 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1397c vapC10 |
ribonuclease VapC10 | 452 | 452 ctx | neighborhood:452 |
Rv1398c vapB10 |
antitoxin VapB10 | 452 | 452 ctx | neighborhood:452 |
Rv0198c zmp1 |
zinc metalloprotease | 407 | 407 | |
Rv1146 mmpL13b |
transmembrane transport protein | 453 | 71 | textmining:436 |
Rv2190c ripC |
endopeptidase | 412 | 43 | textmining:411 |
Rv3475 |
Possible transposase for insertion element IS6110 [second part]; Rv3475, (MTCY13E12.28), len: 328 aa. Probable transposase subunit for IS611 | 654 | 41 | textmining:654 |
Rv3474 |
Possible transposase for insertion element IS6110 (fragment); Rv3474, (MTCY13E12.27), len: 108 aa. Probable transposase subunit for IS6110. | 654 | 41 | textmining:654 |
Rv3844 |
transposase | 654 | 41 | textmining:654 |
Rv1115 hyp |
hypothetical protein | 652 | 41 | textmining:652 |
Rv3348 |
transposase | 627 | 41 | textmining:627 |
Rv0878c PPE13 |
PPE family protein PPE13 | 549 | 41 | textmining:549 |
Rv0388c PPE9 |
Rv0388c, (MTV036.23c), len: 180 aa. PPE9, Member of the Mycobacterium tuberculosis PPE family, highly similar to others e.g. MTCY10G2_10|Z92 | 510 | 41 | textmining:510 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): PE-PGRS family protein PE_PGRS25
- Pfam (hmmscan --cut_ga): PE PF00934.26 (E=4e-34), PGRS PF21526.3 (E=5e-13)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq YP_177809.1)
- Domains: Pfam-A via hmmscan --cut_ga — PE (PF00934.26), PGRS (PF21526.3)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0657 - Curated reference: UniProt P71664 (TrEMBL, unreviewed; Predicted)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
12 functional partner(s); context anchor
vapC10 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv1396c|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