mmpL10 Resolved · high auto-curated
H37Rv Rv1183 · MTBC0 mtbc0_001271 ·
1017 aa · 1329917–1332970 (+) ·
RefSeq NP_215699.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | transmembrane transport protein MmpL10 |
|---|---|
| MTBC0 PGAP re-annotation | RND transporter MmpL10 |
| Revised (this work) | RND transporter MmpL10. Pfam: MMPL (PF03176.22). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WJU1
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Acyltrehalose exporter MmpL10 |
| Curated function | Required for the biosynthesis of polyacyltrehalose (PAT) and the transport of diacyltrehalose (DAT) and possibly PAT to the cell surface. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| Preferred name | mmpL10 |
| eggNOG description | transport protein |
| Orthologous group | COG2409 |
| KEGG orthology |
K06994
|
| Gene Ontology (14) |
GO:0005575, GO:0005576, GO:0005618, GO:0005622, GO:0005623, GO:0005737, GO:0005829, GO:0005886, GO:0016020, GO:0030312, GO:0044424, GO:0044444 +2 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 1.942 · diversifying/relaxed |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 4 synonymous, 22 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
MMPL | PF03176.22 | 5.5e-111 | 45–372 | MMPL family |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: papA3 (acyltransferase papA3), high confidence from genomic context alone (score 948 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1182 papA3 |
acyltransferase papA3 | 992 | 948 ctx | neighborhood:628 coexpression:806 textmining:858 |
Rv1181 pks4 |
polyketide beta-ketoacyl synthase | 968 | 900 ctx | neighborhood:475 coexpression:797 textmining:700 |
Rv3823c mmpL8 |
integral membrane transport protein MmpL8 | 813 | 808 | coexpression:803 |
Rv1184c chp2 hyp |
hypothetical protein | 850 | 747 | coexpression:733 textmining:434 |
Rv3492c |
Mce associated protein | 740 | 740 | coexpression:740 |
Rv1180 pks3 |
polyketide beta-ketoacyl synthase | 826 | 632 | textmining:548 |
Rv3824c papA1 |
acyltransferase | 848 | 605 | coexpression:404 textmining:632 |
Rv1598c hyp |
hypothetical protein | 564 | 564 ctx | cooccurence:563 |
Rv1145 mmpL13a |
transmembrane transport protein | 549 | 494 ctx | cooccurence:491 |
Rv1303 hyp |
hypothetical protein | 485 | 485 ctx | cooccurence:485 |
Rv3825c pks2 |
phthioceranic/hydroxyphthioceranic acid synthase | 666 | 421 | textmining:448 |
Rv3479 |
transmembrane protein | 414 | 415 | coexpression:415 |
Rv3820c papA2 |
trehalose-2-sulfate acyltransferase | 750 | 348 | textmining:632 |
Rv2942 mmpL7 |
transmembrane transport protein MmpL7 | 449 | 317 | |
Rv0383c ttfA hyp |
hypothetical protein | 412 | 284 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: transmembrane transport protein MmpL10
- MTBC0 PGAP product: RND transporter MmpL10
- Pfam (hmmscan --cut_ga): MMPL PF03176.22 (E=5e-111)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215699.1)
- Domains: Pfam-A via hmmscan --cut_ga — MMPL (PF03176.22)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG2409 - Curated reference: UniProt P9WJU1 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
19 functional partner(s); context anchor
papA3 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001271|Rv1183|mmpL10 MFARLGELVARRPWVVVGCWVALALVLPMAVPSLAEMAQRHPVAVLPADAPSSVAVRQMAEAFHESGSENILVVLLTDEKGLGAADENVYHTLVDRLRNDAKDVVMLQDFLTTPPLREVLGSKDGKAWILPIGLAGDLGTPKSYHAYTDVERIVKRTVAGTTLTANVTGPAATVADLTDAGARDRASIELAIAVMLLVILMVIYRNPVTMLLPLVTIGASLMTAQALVAGVSLVGGLAVSNQAIVLLSAMIAGAGTDYAVFLISRYHEYVRLGEHPERAVQRAMMSVGKVIAASAATVGITFLGMRFAKLGVFSTVGPALAIGIAVSFLAAVTLLPAILVLASPRGWVAPRGERMATFWRRAGTRIVRRPKAYLGASLIGLVALASCASLAHFNYDDRKQLPPSDPSSVGYAAMEHHFSVNQTIPEYLIIHSAHDLRTPRGLADLEQLAQRVSQIPGVAMVRGVTRPNGETLEQARATYQAGQVGNRLGGASRMIDERTGDLNRLASGANLLADNLGDVRGQVSRAVAGVRSLVDALAYIQNQFGGNKTFNEIDNAARLVSNIHALGDALQVNFDGIANSFDWLDSVVAALDTSPVCDSNPMCGNARVQFHKLQTARDNGTLDKVVGLARQLQSTRSPQTVSAVVNDLGRSLNSVVRSLKSLGLDNPDAARARLISMQNGANDLASAGRQVADGVQMLVDQTKNMGIGLNQASAFLMAMGNDASQPSMAGFNVPPQVLKSEEFKKVAQAFISPDGHTVRYFIQTDLNPFSTAAMDQVNTIIDTAKGAQPNTSLADASISMSGYPVMLRDIRDYYERDMRLIVAVTVVVVILILMALLRAIVAPLYLVGSVVISYMSAIGLGVVVFQVFLGQELHWSVPGLAFVVLVAVGADYNMLLASRLRDESALGVRSSVIRTVRCTGGVITAAGLIFAASMSGLLFSSIGTVVQGGFIIGVGILIDTFVVRTITVPAMATLLGRASWWPGHPWQRCAPEEGQMSARMSARTKTVFQAVADGSKR