mmpL7 Resolved · high auto-curated
H37Rv Rv2942 · MTBC0 mtbc0_003125 ·
920 aa · 3306067–3308829 (+) ·
RefSeq NP_217458.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | transmembrane transport protein MmpL7 |
|---|---|
| MTBC0 PGAP re-annotation | phthiocerol dimycocerosate RND transporter MmpL7 |
| Revised (this work) | Phthiocerol dimycocerosate RND transporter MmpL7. Pfam: MMPL (PF03176.22). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WJU7
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Phthiocerol dimycocerosate exporter MmpL7 |
| Curated function | Required for export of phthiocerol dimycocerosate (PDIM) to the cell wall. Essential for normal replication during the active-growth phase of the murine tuberculosis model..; FUNCTION: Confers a high level of resistance to isoniazid (INH) when overexpressed in M.smegmatis. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
N Cell motilityT Signal transduction mechanisms
|
|---|---|
| Preferred name | mmpL7 |
| eggNOG description | involved in translocation of phthiocerol dimycocerosate (DIM) in the cell wall |
| Orthologous group | COG2409 |
| KEGG orthology |
K06994
|
| Gene Ontology (44) |
GO:0005575, GO:0005623, GO:0005886, GO:0005887, GO:0006810, GO:0006855, GO:0006869, GO:0008150, GO:0009605, GO:0009607, GO:0010876, GO:0015893 +32 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.234 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 13 synonymous, 8 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
MMPL | PF03176.22 | 4.7e-70 | 75–382 | MMPL family |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: fadD28 (long-chain-fatty-acid--AMP ligase FadD28), high confidence from genomic context alone (score 818 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2941 fadD28 |
long-chain-fatty-acid--AMP ligase FadD28 | 937 | 818 ctx | neighborhood:801 textmining:669 |
Rv2608 PPE42 |
PPE family protein PPE42 | 769 | 769 ctx | cooccurence:768 |
Rv3343c PPE54 |
PPE family protein PPE54 | 764 | 765 ctx | cooccurence:758 |
Rv2945c lppX |
lipoprotein LppX | 953 | 764 ctx | cooccurence:760 textmining:812 |
Rv1917c PPE34 |
PPE family protein PPE34 | 760 | 761 ctx | cooccurence:759 |
Rv0355c PPE8 |
PPE family protein PPE8 | 758 | 759 ctx | cooccurence:756 |
Rv0442c PPE10 |
PPE family protein PPE10 | 758 | 758 ctx | cooccurence:756 |
Rv3347c PPE55 |
PPE family protein PPE55 | 757 | 758 ctx | cooccurence:754 |
Rv2209 |
integral membrane protein | 757 | 758 ctx | cooccurence:741 |
Rv3350c PPE56 |
PPE family protein PPE56 | 755 | 755 ctx | cooccurence:753 |
Rv0305c PPE6 |
PPE family protein PPE6 | 753 | 754 ctx | cooccurence:753 |
Rv0878c PPE13 |
PPE family protein PPE13 | 753 | 754 ctx | cooccurence:749 |
Rv1004c |
membrane protein | 742 | 743 ctx | cooccurence:742 |
Rv1753c PPE24 |
PPE family protein PPE24 | 739 | 740 ctx | cooccurence:738 |
Rv1548c PPE21 |
PPE family protein PPE21 | 739 | 740 ctx | cooccurence:738 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: transmembrane transport protein MmpL7
- MTBC0 PGAP product: phthiocerol dimycocerosate RND transporter MmpL7
- Pfam (hmmscan --cut_ga): MMPL PF03176.22 (E=5e-70)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_217458.1)
- Domains: Pfam-A via hmmscan --cut_ga — MMPL (PF03176.22)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG2409 - Curated reference: UniProt P9WJU7 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
125 functional partner(s); context anchor
fadD28 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_003125|Rv2942|mmpL7 MPSPAGRLHRIRYIRLKKSSPDCRATITSGSADGQRRSPRLTNLLVVAAWVAAAVIANLLLTFTQAEPHDTSPALLPQDAKTAAATSRIAQAFPGTGSNAIAYLVVEGGSTLEPQDQPYYDAAVGALRADTRHVGSVLDWWSDPVTAPLGTSPDGRSATAMVWLRGEAGTTQAAESLDAVRSVLRQLPPSEGLRASIVVPAITNDMPMQITAWQSATIVTVAAVIAVLLLLRARLSVRAAAIVLLTADLSLAVAWPLAAVVRGHDWGTDSVFSWTLAAVLTIGTITAATMLAARLGSDAGHSAAPTYRDSLPAFALPGACVAIFTGPLLLARTPALHGVGTAGLGVFVALAASLTVLPALIALAGASRQLPAPTTGAGWTGRLSLPVSSASALGTAAVLAICMLPIIGMRWGVAENPTRQGGAQVLPGNALPDVVVIKSARDLRDPAALIAINQVSHRLVEVPGVRKVESAAWPAGVPWTDASLSSAAGRLADQLGQQAGSFVPAVTAIKSMKSIIEQMSGAVDQLDSTVNVTLAGARQAQQYLDPMLAAARNLKNKTTELSEYLETIHTWIVGFTNCPDDVLCTAMRKVIEPYDIVVTGMNELSTGADRISAISTQTMSALSSAPRMVAQMRSALAQVRSFVPKLETTIQDAMPQIAQASAMLKNLSADFADTGEGGFHLSRKDLADPSYRHVRESMFSSDGTATRLFLYSDGQLDLAAAARAQQLEIAAGKAMKYGSLVDSQVTVGGAAQIAAAVRDALIHDAVLLAVILLTVVALASMWRGAVHGAAVGVGVLASYLAALGVSIALWQHLLDRELNALVPLVSFAVLASCGVPYLVAGIKAGRIADEATGARSKGAVSGRGAVAPLAALGGVFGAGLVLVSGGSFSVLSQIGTVVVLGLGVLITVQRAWLPTTPGRR