Rv0051 Family assigned · medium auto-curated
H37Rv Rv0051 · MTBC0 mtbc0_000056 ·
560 aa · 55804–57486 (+) ·
RefSeq NP_214565.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | transmembrane protein |
|---|---|
| MTBC0 PGAP re-annotation | glycosyltransferase family 87 protein |
| Revised (this work) | Glycosyltransferase family 87 protein. Pfam: GT87 (PF09594.17). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P71708
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Probable conserved transmembrane protein |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| eggNOG description | integral membrane protein |
| Orthologous group | COG5650 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.41 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 7 synonymous, 6 missense, 2 nonsense, 0 frameshift |
| Disruption | 2 distinct premature-stop/frameshift site(s); most common in 0.41% of strains (589) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
GT87 | PF09594.17 | 7.0e-15 | 181–407 | Glycosyltransferase family 87 |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: ponA1 (bifunctional penicillin-insensitive transglycosylase/penicillin-sensitive transpeptidase), high confidence from genomic context alone (score 908 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0052 hyp |
hypothetical protein | 937 | 938 ctx | neighborhood:722 coexpression:785 |
Rv0050 ponA1 |
bifunctional penicillin-insensitive transglycosylase/penicillin-sensitive transpeptidase | 908 | 908 ctx | neighborhood:882 |
Rv1159 pimE |
polyprenol-phosphate-mannose-dependent alpha-(1-2)-phosphatidylinositol pentamannoside mannosyltransferase | 865 | 736 ctx | cooccurence:734 textmining:510 |
Rv0955 |
integral membrane protein | 722 | 722 ctx | cooccurence:718 |
Rv3604c |
transmembrane protein | 716 | 717 ctx | cooccurence:714 |
Rv0053 rpsF |
30S ribosomal protein S6 | 708 | 708 ctx | neighborhood:708 |
Rv1402 priA |
primosomal protein N' | 696 | 696 ctx | cooccurence:693 |
Rv0049 hyp |
hypothetical protein | 682 | 682 ctx | neighborhood:544 |
Rv1459c mptB |
alpha-(1->6)-mannopyranosyltransferase | 662 | 613 ctx | cooccurence:599 |
Rv0054 ssb |
single-strand DNA-binding protein | 613 | 613 ctx | neighborhood:611 |
Rv3912 rsmA |
anti-sigma-M factor RsmA | 606 | 606 ctx | cooccurence:606 |
Rv3118 sseC1 hyp |
hypothetical protein | 588 | 588 ctx | cooccurence:582 |
Rv0814c sseC2 hyp |
hypothetical protein | 584 | 585 ctx | cooccurence:582 |
Rv2181 |
alpha-(1-2)-phosphatidylinositol mannoside mannosyltransferase | 601 | 555 ctx | cooccurence:552 |
Rv3304 hyp |
hypothetical protein | 519 | 520 ctx | cooccurence:518 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: transmembrane protein
- MTBC0 PGAP product: glycosyltransferase family 87 protein
- Pfam (hmmscan --cut_ga): GT87 PF09594.17 (E=7e-15)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_214565.1)
- Domains: Pfam-A via hmmscan --cut_ga — GT87 (PF09594.17)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG5650 - Curated reference: UniProt P71708 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
41 functional partner(s); context anchor
ponA1 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_000056|Rv0051| MTGALSQSSNISPLPLAADLRSADNRDCPSRTDVLGAALANVVGGPVGRHALIGRTRLMTPLRVMFAIALVFLALGWSTKAACLQSTGTGPGDQRVANWDNQRAYYQLCYSDTVPLYGAELLSQGKFPYKSSWIETDSNGTPQLRYDGQIAVRYMEYPVLTGIYQYLSMAIAKTYTALSKVAPLPVVAEVVMFFNVAAFGLALAWLTTVWATSGLAGRRIWDAALVAASPLVIFQIFTNFDALATGLATSGLLAWARRRPVLAGVLIGLGSAAKLYPLLFLYPLLLLGIRAGRLNALARTMAAAAATWLLVNLPVMLLFPRGWSEFFRLNTRRGDDMDSLYNVVKSFTGWRGFDPTLGFWEPPLVLNTVVTLLFVLCCAAIAYIALTAPHRPRVAQLTFLTVASFLLVNKVWSPQFSLWLVPLAVLALPHRRILLAWMTIDALVWVPRMYYLYGNPSRSLPEQWFTTTVLLRDIAVMVLCGLVVWQIYRPGRDLVRTGGPGALPACGGVDDPVGGVFANAADAPPGRLPSWLRPRLGDEHARERTPDAGRDRTFSGQHRA