desA1 Resolved · high auto-curated
H37Rv Rv0824c · MTBC0 - ·
338 aa · 917734–918750 (-) ·
RefSeq YP_177758.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | acyl-ACP desaturase DesA |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | Acyl-ACP desaturase DesA. Pfam: FA_desaturase_2 (PF03405.21). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
P9WNZ7
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Putative acyl-[acyl-carrier-protein] desaturase DesA1 |
| EC (curated) |
EC 1.14.19.-
|
| Curated function | May be a desaturase involved in mycobacterial fatty acid biosynthesis. |
UniProt still lists this protein as Putative acyl-[acyl-carrier-protein] desaturase DesA1; the revised annotation above is ahead of the current UniProt record.
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
F Nucleotide transport and metabolism
|
|---|---|
| Preferred name | desA1 |
| eggNOG description | desaturase |
| Orthologous group | COG0208 |
| EC number |
EC 1.14.19.11, EC 1.14.19.2, EC 1.14.19.26
|
| KEGG orthology |
K03921
|
| KEGG pathways |
map00061, map01040, map01212
|
| Gene Ontology (29) |
GO:0005575, GO:0005618, GO:0005622, GO:0005623, GO:0005737, GO:0005829, GO:0005886, GO:0008150, GO:0009605, GO:0009607, GO:0016020, GO:0030312 +17 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.048 · strong purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 6 synonymous, 1 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
FA_desaturase_2 | PF03405.21 | 4.5e-86 | 7–311 | Fatty acid desaturase |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: dusB (tRNA-dihydrouridine synthase), high confidence from genomic context alone (score 956 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1094 desA2 |
acyl-ACP desaturase DesA | 989 | 986 | coexpression:843 database:900 |
Rv0823c dusB |
tRNA-dihydrouridine synthase | 979 | 956 ctx | neighborhood:781 coexpression:806 textmining:560 |
Rv3230c |
stearoyl-CoA 9-desaturase electron transfer protein | 931 | 928 | database:900 |
Rv3229c desA3 |
stearoyl-CoA 9-desaturase | 974 | 924 | database:900 textmining:679 |
Rv2524c fas |
fatty acid synthase | 931 | 905 | database:900 |
Rv3052c nrdI |
NrdI protein | 845 | 833 | coexpression:436 experimental:707 |
Rv1177 fdxC |
ferredoxin FdxC | 896 | 798 | coexpression:798 textmining:511 |
Rv3051c nrdE |
ribonucleoside-diphosphate reductase subunit alpha | 772 | 758 | coexpression:550 experimental:468 |
Rv0570 nrdZ |
vitamin B12-dependent ribonucleoside-diphosphate reductase | 769 | 755 | coexpression:544 experimental:468 |
Rv0825c hyp |
hypothetical protein | 748 | 748 ctx | neighborhood:747 |
Rv0826 hyp |
hypothetical protein | 715 | 715 ctx | neighborhood:672 |
Rv0822c hyp |
hypothetical protein | 462 | 443 ctx | neighborhood:429 |
Rv0184 hyp |
hypothetical protein | 429 | 430 ctx | cooccurence:423 |
Rv0876c |
transmembrane protein | 428 | 428 ctx | cooccurence:423 |
Rv3629c |
integral membrane protein | 427 | 427 ctx | cooccurence:402 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): acyl-ACP desaturase DesA
- Pfam (hmmscan --cut_ga): FA_desaturase_2 PF03405.21 (E=4e-86)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq YP_177758.1)
- Domains: Pfam-A via hmmscan --cut_ga — FA_desaturase_2 (PF03405.21)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0208 - Curated reference: UniProt P9WNZ7 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
37 functional partner(s); context anchor
dusB - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv0824c|desA1 MSAKLTDLQLLHELEPVVEKYLNRHLSMHKPWNPHDYIPWSDGKNYYALGGQDWDPDQSKLSDVAQVAMVQNLVTEDNLPSYHREIAMNMGMDGAWGQWVNRWTAEENRHGIALRDYLVVTRSVDPVELEKLRLEVVNRGFSPGQNHQGHYFAESLTDSVLYVSFQELATRISHRNTGKACNDPVADQLMAKISADENLHMIFYRDVSEAAFDLVPNQAMKSLHLILSHFQMPGFQVPEFRRKAVVIAVGGVYDPRIHLDEVVMPVLKKWRIFEREDFTGEGAKLRDELALVIKDLELACDKFEVSKQRQLDREARTGKKVSAHELHKTAGKLAMSRR