Rv0369c Resolved · high auto-curated
H37Rv Rv0369c · MTBC0 - ·
171 aa · 446531–447046 (-) ·
RefSeq NP_214883.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | membrane oxidoreductase |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | Membrane oxidoreductase. Pfam: COXG (PF06240.20). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
O53704
TrEMBL · unreviewed
· Predicted
|
|---|---|
| UniProt name | Possible membrane oxidoreductase |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| eggNOG description | carbon monoxide dehydrogenase subunit G |
| Orthologous group | COG3427 |
| KEGG orthology |
K09386
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.086 · strong purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 4 synonymous, 1 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
COXG | PF06240.20 | 6.4e-13 | 1–116 | Carbon monoxide dehydrogenase subunit G (CoxG) |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv0373c (carbon monoxyde dehydrogenase large subunit), high confidence from genomic context alone (score 936 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0368c hyp |
hypothetical protein | 971 | 972 ctx | neighborhood:882 cooccurence:768 |
Rv0373c |
carbon monoxyde dehydrogenase large subunit | 954 | 936 ctx | neighborhood:760 cooccurence:735 |
Rv0375c |
carbon monoxyde dehydrogenase medium subunit | 938 | 935 ctx | neighborhood:754 cooccurence:730 |
Rv0372c hyp |
hypothetical protein | 925 | 926 ctx | neighborhood:773 cooccurence:684 |
Rv0376c hyp |
hypothetical protein | 898 | 898 ctx | neighborhood:700 cooccurence:672 |
Rv0370c |
oxidoreductase | 972 | 895 ctx | neighborhood:773 cooccurence:551 textmining:751 |
Rv0374c |
carbon monoxyde dehydrogenase small subunit | 841 | 841 ctx | neighborhood:760 |
Rv0377 |
HTH-type transcriptional regulator | 815 | 815 ctx | neighborhood:663 cooccurence:472 |
Rv0371c hyp |
hypothetical protein | 801 | 802 ctx | neighborhood:773 |
Rv0365c hyp |
hypothetical protein | 744 | 744 ctx | neighborhood:742 |
Rv0367c hyp |
hypothetical protein | 609 | 608 ctx | neighborhood:606 |
Rv0366c hyp |
hypothetical protein | 537 | 537 ctx | neighborhood:534 |
Rv2426c hyp |
hypothetical protein | 495 | 496 ctx | cooccurence:434 |
Rv0382c pyrE |
orotate phosphoribosyltransferase | 702 | 179 | textmining:653 |
Rv2250A |
Possible flavoprotein; Rv2250A, len: 139 aa. Conserved hypothetical protein, possibly flavoprotein. Similar to N-terminus of SCF91.28c|AL132 | 783 | 166 | textmining:751 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): membrane oxidoreductase
- Pfam (hmmscan --cut_ga): COXG PF06240.20 (E=6e-13)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_214883.1)
- Domains: Pfam-A via hmmscan --cut_ga — COXG (PF06240.20)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG3427 - Curated reference: UniProt O53704 (TrEMBL, unreviewed; Predicted)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
24 functional partner(s); context anchor
Rv0373c - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv0369c| MPGAQLIGHEGDEYLGKVKVKVGPVTSEFSGKVHFVEQDRNQHRAVFDAKGKEARGTGNAAATVAAQLHEVGERTRVTVDTDLKIVGKLAQFGSGMLQQVSEKLLGQFVDSLEAELAAQSSESPQGTPPATEAAPIDLLQLADGGQLKKYGSALLAALTVLLLIWVLRRRR