mntH Resolved · high auto-curated
H37Rv Rv0924c · MTBC0 - ·
428 aa · 1030578–1031864 (-) ·
RefSeq YP_177767.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | divalent metal cation transporter MntH |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | Divalent metal cation transporter MntH. Pfam: Nramp (PF01566.26). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
P9WIZ5
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Divalent metal cation transporter MntH |
| Curated function | H(+)-stimulated, divalent metal cation uptake system. Transports zinc and iron. Can also interact with manganese and copper. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
P Inorganic ion transport and metabolism
|
|---|---|
| Preferred name | mntH |
| eggNOG description | H( )-stimulated, divalent metal cation uptake system |
| Orthologous group | COG1914 |
| KEGG orthology |
K03322
|
| Gene Ontology (26) |
GO:0000041, GO:0006810, GO:0006811, GO:0006812, GO:0006826, GO:0006828, GO:0006829, GO:0008150, GO:0030001, GO:0034220, GO:0034755, GO:0051179 +14 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 1.14 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 3 synonymous, 9 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Nramp | PF01566.26 | 1.1e-125 | 39–423 | Natural resistance-associated macrophage protein-like |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv0927c (oxidoreductase), medium confidence from genomic context alone (score 448 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0923c hyp |
hypothetical protein | 887 | 887 ctx | neighborhood:882 |
Rv0925c hyp |
hypothetical protein | 722 | 722 ctx | neighborhood:708 |
Rv2060 |
integral membrane protein | 855 | 658 | coexpression:647 textmining:595 |
Rv1305 atpE |
ATP synthase subunit C | 616 | 603 | database:557 |
Rv0926c hyp |
hypothetical protein | 561 | 561 ctx | neighborhood:549 |
Rv0264c hyp |
hypothetical protein | 509 | 491 | coexpression:405 |
Rv0263c hyp |
hypothetical protein | 515 | 489 | coexpression:403 |
Rv0908 ctpE |
metal cation transporter ATPase E | 645 | 480 | |
Rv0425c ctpH |
metal cation transporting ATPase H | 522 | 477 | |
Rv0107c ctpI |
cation-transporter ATPase I | 660 | 474 | |
Rv1997 ctpF |
cation transporter ATPase F | 519 | 474 | |
Rv1249c |
membrane protein | 490 | 459 | coexpression:414 |
Rv1488 hyp |
hypothetical protein | 449 | 450 | database:448 |
Rv0927c |
oxidoreductase | 454 | 448 ctx | neighborhood:423 |
Rv2788 sirR |
transcriptional repressor SirR | 674 | 436 | textmining:446 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): divalent metal cation transporter MntH
- Pfam (hmmscan --cut_ga): Nramp PF01566.26 (E=1e-125)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq YP_177767.1)
- Domains: Pfam-A via hmmscan --cut_ga — Nramp (PF01566.26)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1914 - Curated reference: UniProt P9WIZ5 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
29 functional partner(s); context anchor
Rv0927c - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv0924c|mntH MAGEFRLLSHLCSRGSKVGELAQDTRTSLKTSWYLLGPAFVAAIAYVDPGNVAANVSSGAQFGYLLLWVIVAANVMAALVQYLSAKLGLVTGRSLPEAIGKRMGRPARLAYWAQAEIVAMATDVAEVIGGAIALRIMFNLPLPIGGIITGVVSLLLLTIQDRRGQRLFERVITALLLVIAIGFTASFFVVTPPPNAVLGGLAPRFQGTESVLLAAAIMGATVMPHAVYLHSGLARDRHGHPDPGPQRRRLLRVTRWDVGLAMLIAGGVNAAMLLVAALNMRGRGDTASIEGAYHAVHDTLGATIAVLFAVGLLASGLASSSVGAYAGAMIMQGLLHWSVPMLVRRLITLGPALAILTLGFDPTRTLVLSQVVLSFGIPFAVLPLVKLTGSPAVMGGDTNHRATTWVGWVVAVMVSLLNVMLIYLTVTG