Rv0276 Family assigned · medium auto-curated
H37Rv Rv0276 · MTBC0 mtbc0_000293 ·
306 aa · 332130–333050 (+) ·
RefSeq NP_214790.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | hypothetical protein |
|---|---|
| MTBC0 PGAP re-annotation | oxygenase MpaB family protein |
| Revised (this work) | Oxygenase MpaB family protein. Pfam: MPAB_Lcp_cat (PF09995.16). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
O53682
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | ER-bound oxygenase mpaB/mpaB'/Rubber oxygenase catalytic domain-containing protein |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| eggNOG description | protein conserved in bacteria |
| Orthologous group | COG3662 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.159 · strong purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 15 synonymous, 6 missense, 1 nonsense, 0 frameshift |
| Disruption | 1 distinct premature-stop/frameshift site(s); most common in 0.41% of strains (600) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
MPAB_Lcp_cat | PF09995.16 | 1.1e-66 | 44–274 | ER-bound oxygenase mpaB/B'/Rubber oxygenase, catalytic domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv0275c (transcriptional regulator), high confidence from genomic context alone (score 847 excluding text-mining). This association is the citable seed of a function hypothesis for this hypothetical protein.
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0275c |
transcriptional regulator | 968 | 847 ctx | neighborhood:599 cooccurence:629 textmining:803 |
Rv0825c hyp |
hypothetical protein | 623 | 623 ctx | cooccurence:572 |
Rv1645c hyp |
hypothetical protein | 526 | 526 ctx | cooccurence:526 |
Rv1683 |
bifunctional long-chain acyl-CoA synthase/lipase | 454 | 454 ctx | cooccurence:451 |
Rv1132 hyp |
hypothetical protein | 448 | 448 ctx | cooccurence:448 |
Rv0826 hyp |
hypothetical protein | 412 | 413 ctx | cooccurence:411 |
Rv1846c blaI |
transcriptional repressor BlaI | 460 | 347 | |
Rv3830c |
TetR family transcriptional regulator | 718 | 238 | textmining:645 |
Rv3168 |
aminoglycoside phosphotransferase | 810 | 229 | textmining:764 |
Rv3829c |
dehydrogenase | 828 | 162 | textmining:804 |
Rv3082c virS |
HTH-type transcriptional regulator VirS | 446 | 64 | textmining:433 |
Rv2062c cobN |
cobalamin biosynthesis protein CobN | 414 | 46 | textmining:411 |
Rv3167c |
TetR family transcriptional regulator | 646 | 45 | textmining:645 |
Rv3178 |
nitroreductase | 656 | 44 | textmining:655 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: hypothetical protein
- MTBC0 PGAP product: oxygenase MpaB family protein
- Pfam (hmmscan --cut_ga): MPAB_Lcp_cat PF09995.16 (E=1e-66)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_214790.1)
- Domains: Pfam-A via hmmscan --cut_ga — MPAB_Lcp_cat (PF09995.16)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG3662 - Curated reference: UniProt O53682 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
14 functional partner(s); context anchor
Rv0275c - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_000293|Rv0276| MAISLVAHQPIPHVERPMADPPRLQLARRRRSAAGPGGNEDSLMGVALLAGPANVIMELAMPGVGYGVLESRVESGRLDRHPIKRARTTFTYVAVAVAGSDDQKAAFRRAVNKVHAQVYSTPESPVSYHAFDPELQLWVAACLYKGGVDVYRTFVGEMDDEEADHHYRAGMAMGTTLQVPPQMWPPDRAAFDRYWRQSLDRVHIDDVVRDYLYPIVALRIRGIALPGPLRRLSEGIALLITTGFLPQRFRDEMRLPWDATKQRRFDALMAVLRTVNRLMPRFVREFPFNLMLWDLDRRMRRGRPLV