Rv3720 Resolved · high auto-curated
H37Rv Rv3720 · MTBC0 - ·
420 aa · 4163736–4164998 (+) ·
RefSeq NP_218237.3
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | fatty acid synthase |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | Fatty acid synthase. Pfam: CMAS (PF02353.27), Methyltransf_23 (PF13489.13), Methyltransf_25 (PF13649.13), Methyltransf_11 (PF08241.19). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
O69687
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Probable fatty acid methyltransferase Rv3720 |
| EC (curated) |
EC 2.1.1.-
|
| Curated function | May be a S-adenosylmethionine-dependent methyltransferase involved in fatty acid metabolism. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
M Cell wall / membrane / envelope biogenesis
|
|---|---|
| Preferred name | cfa |
| eggNOG description | Cyclopropane-fatty-acyl-phospholipid synthase |
| Orthologous group | COG2230 |
| EC number |
EC 2.1.1.79
|
| KEGG orthology |
K00574
|
| Gene Ontology (8) |
GO:0005575, GO:0005618, GO:0005623, GO:0005886, GO:0016020, GO:0030312, GO:0044464, GO:0071944
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.264 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 5 synonymous, 4 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
CMAS | PF02353.27 | 9.4e-101 | 130–398 | Mycolic acid cyclopropane synthetase |
Methyltransf_23 | PF13489.13 | 5.7e-10 | 184–300 | Methyltransferase domain |
Methyltransf_25 | PF13649.13 | 8.4e-10 | 196–289 | Methyltransferase domain |
Methyltransf_11 | PF08241.19 | 1.2e-09 | 196–291 | Methyltransferase domain |
Functional interaction network (STRING v12, guilt-by-association)
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3719 hyp |
hypothetical protein | 951 | 951 ctx | neighborhood:859 cooccurence:648 |
Rv3718c hyp |
hypothetical protein | 774 | 774 ctx | neighborhood:773 |
Rv0448c hyp |
hypothetical protein | 472 | 451 | |
Rv0447c ufaA1 |
cyclopropane-fatty-acyl-phospholipid synthase UfaA | 400 | 400 | |
Rv3396c guaA |
GMP synthase | 426 | 266 | |
Rv3502c |
3-oxoacyl-ACP reductase | 429 | 72 | textmining:411 |
Rv0242c fabG4 |
3-oxoacyl-ACP reductase FabG | 454 | 71 | textmining:437 |
Rv3254 hyp |
hypothetical protein | 808 | 66 | textmining:803 |
Rv3304 hyp |
hypothetical protein | 639 | 66 | textmining:630 |
Rv2488c |
LuxR family transcriptional regulator | 422 | 63 | textmining:409 |
Rv3253c |
cationic amino acid transport integral membrane protein | 636 | 58 | textmining:630 |
Rv0386 |
transcriptional regulator | 417 | 54 | textmining:409 |
Rv3302c glpD2 |
glycerol-3-phosphate dehydrogenase | 514 | 50 | textmining:510 |
Rv1589 bioB |
biotin synthetase | 442 | 50 | textmining:437 |
Rv0373c |
carbon monoxyde dehydrogenase large subunit | 413 | 48 | textmining:409 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): fatty acid synthase
- Pfam (hmmscan --cut_ga): CMAS PF02353.27 (E=9e-101), Methyltransf_23 PF13489.13 (E=6e-10), Methyltransf_25 PF13649.13 (E=8e-10), Methyltransf_11 PF08241.19 (E=1e-09)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_218237.3)
- Domains: Pfam-A via hmmscan --cut_ga — CMAS (PF02353.27), Methyltransf_23 (PF13489.13), Methyltransf_25 (PF13649.13), Methyltransf_11 (PF08241.19)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG2230 - Curated reference: UniProt O69687 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023, doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 — 21 functional partner(s)
- Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv3720| MAEILEIFTATGQHPLKFTAYDGSTAGQDDATLGLDLRTPRGATYLATAPGELGLARAYVSGDLQAHGVHPGDPYELLKTLTERVDFKRPSARVLANVVRSIGVEHILPIAPPPQEARPRWRRMANGLLHSKTRDAEAIHHHYDVSNNFYEWVLGPSMTYTCAVFPNAEASLEQAQENKYRLIFEKLRLEPGDRLLDVGCGWGGMVRYAARRGVRVIGATLSAEQAKWGQKAVEDEGLSDLAQVRHSDYRDVAETGFDAVSSIGLTEHIGVKNYPFYFGFLKSKLRTGGLLLNHCITRHDNRSTSFAGGFTDRYVFPDGELTGSGRITTEIQQVGLEVLHEENFRHHYAMTLRDWCGNLVEHWDDAVAEVGLPTAKVWGLYMAASRVAFERNNLQLHHVLATKVDPRGDDSLPLRPWWQP