mrsA Resolved · high auto-curated
H37Rv Rv3441c · MTBC0 mtbc0_003660 ·
448 aa · 3885910–3887256 (-) ·
RefSeq NP_217958.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | phosphoglucosamine mutase |
|---|---|
| MTBC0 PGAP re-annotation | phosphoglucosamine mutase |
| Revised (this work) | Phosphoglucosamine mutase. Pfam: PGM_PMM_I (PF02878.23), PGM_PMM_II (PF02879.23), PGM_PMM_III (PF02880.23), PGM_PMM_IV (PF00408.27). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WN41
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Phosphoglucosamine mutase |
| EC (curated) |
EC 5.4.2.10
|
| Curated function | Catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
G Carbohydrate transport and metabolism
|
|---|---|
| Preferred name | glmM |
| eggNOG description | Catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate |
| Orthologous group | COG1109 |
| EC number |
EC 5.4.2.10
|
| KEGG orthology |
K03431
|
| KEGG pathways |
map00520, map01100, map01130
|
| Gene Ontology (2) |
GO:0008150, GO:0040007
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.254 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 6 synonymous, 4 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
PGM_PMM_I | PF02878.23 | 4.7e-42 | 3–133 | Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain I |
PGM_PMM_II | PF02879.23 | 3.6e-16 | 161–256 | Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain II |
PGM_PMM_III | PF02880.23 | 1.1e-29 | 260–369 | Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain III |
PGM_PMM_IV | PF00408.27 | 1.0e-18 | 376–442 | Phosphoglucomutase/phosphomannomutase, C-terminal domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: glmU (bifunctional UDP-N-acetylglucosamine pyrophosphorylase/glucosamine-1-phosphate N-acetyltransferase), high confidence from genomic context alone (score 948 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1018c glmU |
bifunctional UDP-N-acetylglucosamine pyrophosphorylase/glucosamine-1-phosphate N-acetyltransferase | 993 | 948 ctx | cooccurence:465 database:900 textmining:879 |
Rv3436c glmS |
glucosamine--fructose-6-phosphate aminotransferase | 992 | 943 | database:900 textmining:871 |
Rv3332 nagA |
N-acetylglucosamine-6-phosphate deacetylase NagA | 981 | 901 | database:900 textmining:823 |
Rv3440c hyp |
hypothetical protein | 826 | 826 ctx | neighborhood:816 |
Rv3439c hyp |
hypothetical protein | 822 | 822 ctx | neighborhood:816 |
Rv3442c rpsI |
30S ribosomal protein S9 | 782 | 782 ctx | neighborhood:761 |
Rv3443c rplM |
50S ribosomal protein L13 | 734 | 734 ctx | neighborhood:724 |
Rv1421 rapZ hyp |
hypothetical protein | 663 | 646 ctx | cooccurence:630 |
Rv3907c pcnA |
poly(A) polymerase PcnA | 590 | 571 ctx | fusion:552 |
Rv1407 fmu |
16S rRNA m5C967 methyltransferase | 563 | 545 ctx | cooccurence:483 |
Rv2156c murX |
phospho-N-acetylmuramoyl-pentappeptidetransferase | 545 | 516 ctx | cooccurence:493 |
Rv3264c manB |
D-alpha-D-mannose-1-phosphate guanylyltransferase ManB | 534 | 484 | coexpression:424 |
Rv2152c murC |
UDP-N-acetylmuramate--alanine ligase | 749 | 467 | textmining:550 |
Rv2101 helZ |
helicase HelZ | 478 | 458 | database:416 |
Rv1315 murA |
UDP-N-acetylglucosamine 1-carboxyvinyltransferase | 710 | 448 ctx | cooccurence:418 textmining:497 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: phosphoglucosamine mutase
- MTBC0 PGAP product: phosphoglucosamine mutase
- Pfam (hmmscan --cut_ga): PGM_PMM_I PF02878.23 (E=5e-42), PGM_PMM_II PF02879.23 (E=4e-16), PGM_PMM_III PF02880.23 (E=1e-29), PGM_PMM_IV PF00408.27 (E=1e-18)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_217958.1)
- Domains: Pfam-A via hmmscan --cut_ga — PGM_PMM_I (PF02878.23), PGM_PMM_II (PF02879.23), PGM_PMM_III (PF02880.23), PGM_PMM_IV (PF00408.27)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1109 - Curated reference: UniProt P9WN41 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
68 functional partner(s); context anchor
glmU - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_003660|Rv3441c|mrsA MGRLFGTDGVRGVANRELTAELALALGAAAARRLSRSGAPGRRVAVLGRDPRASGEMLEAAVIAGLTSEGVDALRVGVLPTPAVAYLTGAYDADFGVMISASHNPMPDNGIKIFGPGGHKLDDDTEDQIEDLVLGVSRGPGLRPAGAGIGRVIDAEDATERYLRHVAKAATARLDDLAVVVDCAHGAASSAAPRAYRAAGARVIAINAEPNGRNINDGCGSTHLDPLRAAVLAHRADLGLAHDGDADRCLAVDANGDLVDGDAIMVVLALAMKEAGELACNTLVATVMSNLGLHLAMRSAGVTVRTTAVGDRYVLEELRAGDYSLGGEQSGHIVMPALGSTGDGIVTGLRLMTRMVQTGSSLSDLASAMRTLPQVLINVEVVDKATAAAAPSVRTAVEQAAAELGDTGRILLRPSGTEPMIRVMVEAADEGVAQRLAATVADAVSTAR