Rv1815 Family assigned · low
H37Rv Rv1815 · MTBC0 mtbc0_001927 ·
221 aa · 2075539–2076204 (+) ·
RefSeq NP_216331.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | hypothetical protein |
|---|---|
| MTBC0 PGAP re-annotation | hypothetical protein |
| Revised (this work) | Protease-like fold (L5 protease, PDB 5MRT); putative peptidase. |
Curated reference (UniProt)
| UniProt |
P9WLR9
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Uncharacterized protein Rv1815 |
UniProt still lists this protein as Uncharacterized protein Rv1815; the revised annotation above is ahead of the current UniProt record.
Functional vocabulary (eggNOG-mapper, orthology transfer)
| Orthologous group | 29WEC |
|---|---|
| Gene Ontology (7) |
GO:0005575, GO:0005576, GO:0005618, GO:0005623, GO:0030312, GO:0044464, GO:0071944
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.26 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 4 synonymous, 3 missense, 0 nonsense, 3 frameshift |
| Disruption | 3 distinct premature-stop/frameshift site(s); most common in 0.33% of strains (479) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
No Pfam-A domain above the gathering threshold (or not yet scanned).
Structural neighbours (Foldseek on the ESMFold model, exploratory)
ESMFold model confidence: mean pLDDT 95.4 (very high). A confident model makes the fold comparison meaningful.
Best matches against the PDB, ranked by Foldseek homology probability. A high probability / TM-score suggests a shared fold; unless flagged sig (E < 0.01) these are fold hypotheses, not assignments.
| Target | Prob | TM | E-value | Description |
|---|---|---|---|---|
5mrt-assembly1_A |
1.00 | 0.71 | 3.3e-08 sig | 5mrt-assembly1_A Crystal structure of L5 protease Lysobacter sp. XL1 |
5mrt-assembly2_B |
1.00 | 0.70 | 3.0e-08 sig | 5mrt-assembly2_B Crystal structure of L5 protease Lysobacter sp. XL1 |
1gbi-assembly1_A |
1.00 | 0.70 | 5.1e-08 sig | 1gbi-assembly1_A ALPHA-LYTIC PROTEASE WITH MET 190 REPLACED BY ALA AND GLY 216 REPLACED BY LEU COMPLEX WITH METHOXYSUCCINYL-ALA-ALA-PRO-PHENYLALANINE BORONIC ACID |
3urd-assembly1_A |
1.00 | 0.70 | 5.7e-08 sig | 3urd-assembly1_A T181A mutant of alpha-Lytic Protease |
3lpr-assembly1_A |
1.00 | 0.68 | 3.2e-08 sig | 3lpr-assembly1_A STRUCTURAL BASIS FOR BROAD SPECIFICITY IN ALPHA-LYTIC PROTEASE MUTANTS |
1hpg-assembly1_A |
1.00 | 0.70 | 4.9e-08 sig | 1hpg-assembly1_A A glutamic acid specific serine protease utilizes a novel histidine triad in substrate binding |
5mrs-assembly2_B |
1.00 | 0.68 | 4.1e-08 sig | 5mrs-assembly2_B Crystal structure of L1 protease Lysobacter sp. XL1 in complex with AEBSF |
1qrw-assembly1_A |
1.00 | 0.67 | 4.1e-08 sig | 1qrw-assembly1_A CRYSTAL STRUCTURE OF AN ALPHA-LYTIC PROTEASE MUTANT WITH ACCELERATED FOLDING KINETICS, R102H/G134S, PH 8 |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv1816 (HTH-type transcriptional regulator), high confidence from genomic context alone (score 938 excluding text-mining). This association is the citable seed of a function hypothesis for this hypothetical protein.
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1816 |
HTH-type transcriptional regulator | 938 | 938 ctx | neighborhood:700 coexpression:802 |
Rv1883c hyp |
hypothetical protein | 798 | 798 | coexpression:798 |
Rv1884c rpfC |
resuscitation-promoting factor RpfC | 808 | 768 | coexpression:765 |
Rv1814 erg3 |
membrane-bound C-5 sterol desaturase | 745 | 745 ctx | neighborhood:745 |
Rv3668c |
protease | 510 | 42 | textmining:510 |
Rv3036c TB22.2 hyp |
hypothetical protein | 436 | 41 | textmining:436 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- MTBC0 PGAP product: hypothetical protein
- Foldseek best: 5mrt-assembly1_A Crystal structure of L5 protease Lysobacter sp. XL1 (prob 1.00, E=3e-08, TM=0.71)
- (structure-only promotion reviewed by hand, 2026-06-01)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216331.1)
- Domains: Pfam-A via hmmscan --cut_ga — none above threshold
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
29WEC - Curated reference: UniProt P9WLR9 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Model confidence: ESMFold per-residue pLDDT (mean 95.4, very high)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
6 functional partner(s); context anchor
Rv1816 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001927|Rv1815| MVRLVPRAFAATVALLAAGFSPATASADPVLVFPGMEIRQDNHVCTLGYVDPALKIAFTAGHCRGGGAVTSRDYKVIGHLRAFRDNTPSGSTVATHELIADYEAIVLADDVTASNILPSGRALESRPGVVLHPGQAVCHFGVSTGETCGTVESVNNGWFTMSHGVLSEKGDSGGPVYLAPDGGPAQIVGIFNSVWGGFPAAVSWRSTSEQVHADLGVTPLA