Rv1765c Family assigned · medium auto-curated
H37Rv Rv1765c · MTBC0 - ·
365 aa · 1997418–1998515 (-) ·
RefSeq NP_216281.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | hypothetical protein |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | Contains DUF222 (PF02720.23), HNH (PF01844.30) domain(s); putative function inferred from the domain architecture. |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
O06798
TrEMBL · unreviewed
· Inferred from homology
|
|---|---|
| UniProt name | HNH nuclease domain-containing protein |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
V Defense mechanisms
|
|---|---|
| eggNOG description | PFAM HNH endonuclease |
| Orthologous group | COG1403 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
DUF222 | PF02720.23 | 2.1e-76 | 23–306 | Domain of unknown function (DUF222) |
HNH | PF01844.30 | 1.7e-07 | 314–352 | HNH endonuclease |
Functional interaction network (STRING v12, guilt-by-association)
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0515 hyp |
hypothetical protein | 856 | 857 ctx | cooccurence:765 coexpression:415 |
Rv0336 hyp |
hypothetical protein | 819 | 820 ctx | cooccurence:765 |
Rv3776 hyp |
hypothetical protein | 775 | 775 ctx | cooccurence:762 |
Rv2100 hyp |
hypothetical protein | 771 | 771 ctx | cooccurence:759 |
Rv0605 |
IS1536 family serine type transposase | 759 | 759 | coexpression:759 |
Rv2979c |
resolvase | 757 | 757 | coexpression:757 |
Rv0393 hyp |
hypothetical protein | 748 | 748 ctx | cooccurence:745 |
Rv2885c |
transposase | 731 | 731 | coexpression:731 |
Rv0606 |
Possible transposase (fragment); Rv0606, (MTCY19H5.16c), len: 247 aa. Possible truncated transposase for IS_1536 element, highly similar to | 730 | 730 | coexpression:730 |
Rv2721c hyp |
hypothetical protein | 704 | 705 ctx | cooccurence:703 |
Rv1587c hyp |
hypothetical protein | 681 | 681 ctx | cooccurence:677 |
Rv0094c hyp |
hypothetical protein | 667 | 668 ctx | cooccurence:657 |
Rv1702c hyp |
hypothetical protein | 666 | 667 ctx | cooccurence:647 |
Rv3467 hyp |
hypothetical protein | 665 | 665 ctx | cooccurence:657 |
Rv1128c hyp |
hypothetical protein | 661 | 661 ctx | cooccurence:659 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): hypothetical protein
- Pfam (hmmscan --cut_ga): DUF222 PF02720.23 (E=2e-76), HNH PF01844.30 (E=2e-07)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216281.1)
- Domains: Pfam-A via hmmscan --cut_ga — DUF222 (PF02720.23), HNH (PF01844.30)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1403 - Curated reference: UniProt O06798 (TrEMBL, unreviewed; Inferred from homology)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023, doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 — 62 functional partner(s)
- Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv1765c| MSSTATSGAAVVSPAERVEVLFEELAELAGQRNAIDGRIVEIVAELDRDGLWGVTGARSVAGLVAWKMGCSSGNAHTIATVARRLPEFPRCARGMREGRLSLDQVGVIAGRAGEGSDAHYAQLAGVATVNQLRTALKLEPRPEPEPDFRPEPRPSITRSADEQFSCWRIKLPHVEAAKFDAALQSHLDALIAEYKRDHDNSDGVSDQRPPLPGNVEAFLRLVEAGWDAEVARRPHGQHTTVVMHLDVQERAAGLHLGPLLSESERRYLLCDATFEAWFERDGQVIGCGRTTRQINRRLRRALEHRDRTCVVPGCGATRGLHAHHIRHWQDGGATELANLVLVCPYHHRAHHRGLNRPGESGDSLI