Rv2886c Resolved · high auto-curated
H37Rv Rv2886c · MTBC0 - ·
295 aa · 3195545–3196432 (-) ·
RefSeq NP_217402.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | resolvase |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | Resolvase. Pfam: Resolvase (PF00239.27). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
P9WL35
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Uncharacterized protein Rv2886c |
UniProt still lists this protein as Uncharacterized protein Rv2886c; the revised annotation above is ahead of the current UniProt record.
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
L Replication, recombination and repair
|
|---|---|
| eggNOG description | resolvase |
| Orthologous group | COG2452 |
| KEGG orthology |
K07450
|
| Gene Ontology (11) |
GO:0005575, GO:0005622, GO:0005623, GO:0005737, GO:0005829, GO:0005886, GO:0016020, GO:0044424, GO:0044444, GO:0044464, GO:0071944
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.494 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 3 synonymous, 4 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Resolvase | PF00239.27 | 6.5e-16 | 153–291 | Resolvase, N terminal domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv2885c (transposase), high confidence from genomic context alone (score 947 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2885c |
transposase | 948 | 947 ctx | neighborhood:781 cooccurence:758 |
Rv0922 tnpB |
transposase | 786 | 781 ctx | cooccurence:772 |
Rv3827c |
transposase | 780 | 775 ctx | cooccurence:760 |
Rv2978c tnpB |
transposase | 777 | 771 ctx | cooccurence:757 |
Rv2791c tnpB |
transposase | 775 | 769 ctx | cooccurence:758 |
Rv0606 |
Possible transposase (fragment); Rv0606, (MTCY19H5.16c), len: 247 aa. Possible truncated transposase for IS_1536 element, highly similar to | 775 | 769 ctx | cooccurence:755 |
Rv2887 |
HTH-type transcriptional regulator | 573 | 573 ctx | neighborhood:572 |
Rv1541c lprI |
lipoprotein LprI | 400 | 401 ctx | cooccurence:400 |
Rv2956 hyp |
hypothetical protein | 601 | 156 | textmining:547 |
Rv3334 |
MerR family transcriptional regulator | 590 | 44 | textmining:589 |
Rv0401 |
transmembrane protein | 586 | 44 | textmining:585 |
Rv2566 hyp |
hypothetical protein | 580 | 42 | textmining:580 |
Rv1376 hyp |
hypothetical protein | 411 | 42 | textmining:411 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): resolvase
- Pfam (hmmscan --cut_ga): Resolvase PF00239.27 (E=7e-16)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_217402.1)
- Domains: Pfam-A via hmmscan --cut_ga — Resolvase (PF00239.27)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG2452 - Curated reference: UniProt P9WL35 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
13 functional partner(s); context anchor
Rv2885c - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv2886c| MSRILTHVPGRTVNRSYALPALVGSAAGRLSGNHSHGREAYIALPQWACSRQPSTPPLQTPGRINALWSLRPVLPMPGRGCQLLRLGGRWLSVVCCRNGSMNLVVWAEGNGVARVIAYRWLRVGRLPVPARRVGRVILVDEPAGQPGRWGRTAVCARLSSADQKVDLDRQVVGVTAWATAEQIPVGKVVTEVGSALYGRRRTFLTLLGDPTVRRIVMKRRDRLGRFGFECVQAVLAADGRELVVVDSADVDDDVVGDITEILTSICARLYGKRAAGNRAARAVAAAARAGGHEAR