PE_PGRS55 Family assigned · medium auto-curated
H37Rv Rv3511 · MTBC0 - ·
714 aa · 3939617–3941761 (+) ·
RefSeq YP_177980.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | PE-PGRS family protein PE_PGRS55 |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | PE-PGRS family protein PE_PGRS55. Pfam: PE (PF00934.26), PGRS (PF21526.3). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
Q6MWW8
TrEMBL · unreviewed
· Predicted
|
|---|---|
| UniProt name | PE-PGRS family protein PE_PGRS55 |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
I Lipid transport and metabolism
|
|---|---|
| eggNOG description | Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see |
| Orthologous group | COG0657 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 1.409 · diversifying/relaxed |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 4 synonymous, 14 missense, 0 nonsense, 5 frameshift |
| Disruption | 5 distinct premature-stop/frameshift site(s); most common in 1.80% of strains (2611) · convergent |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
PE | PF00934.26 | 1.5e-33 | 4–94 | PE family |
PGRS | PF21526.3 | 5.7e-11 | 116–184 | PGRS repeats |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: PE_PGRS56 (PE-PGRS family protein PE_PGRS56), high confidence from genomic context alone (score 773 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3512 PE_PGRS56 |
PE-PGRS family protein PE_PGRS56 | 851 | 773 ctx | neighborhood:773 |
Rv0198c zmp1 |
zinc metalloprotease | 407 | 407 | |
Rv3565 aspB |
aspartate aminotransferase AspB | 555 | 96 | textmining:528 |
Rv3538 |
dehydrogenase | 517 | 41 | textmining:517 |
Rv1089 PE10 |
PE family protein PE10; Together with PE9, induces macrophage apoptosis through human Toll-like receptor 4 (TLR4) signaling pathway. Interac | 439 | 41 | textmining:439 |
Rv3551 |
CoA-transferase subunit alpha | 438 | 41 | textmining:438 |
Rv2126c PE_PGRS37 |
PE-PGRS family protein PE_PGRS37 | 437 | 41 | textmining:437 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): PE-PGRS family protein PE_PGRS55
- Pfam (hmmscan --cut_ga): PE PF00934.26 (E=2e-33), PGRS PF21526.3 (E=6e-11)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq YP_177980.1)
- Domains: Pfam-A via hmmscan --cut_ga — PE (PF00934.26), PGRS (PF21526.3)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0657 - Curated reference: UniProt Q6MWW8 (TrEMBL, unreviewed; Predicted)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
7 functional partner(s); context anchor
PE_PGRS56 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv3511|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