mesT Resolved · high auto-curated
H37Rv Rv3176c · MTBC0 - ·
318 aa · 3544344–3545300 (-) ·
RefSeq YP_177938.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | epoxide hydrolase MesT |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | Epoxide hydrolase MesT. Pfam: Abhydrolase_6 (PF12697.14), Abhydrolase_1 (PF00561.27). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
Q6MX03
TrEMBL · unreviewed
· Predicted
|
|---|---|
| UniProt name | Probable epoxide hydrolase MesT |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| Preferred name | mesT |
| eggNOG description | Alpha beta hydrolase |
| Orthologous group | COG0596 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains) pseudogene candidate
| pN/pS | 0.464 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 3 synonymous, 4 missense, 0 nonsense, 1 frameshift |
| Disruption | 1 distinct premature-stop/frameshift site(s); most common in 10.10% of strains (14663) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Abhydrolase_6 | PF12697.14 | 2.4e-12 | 52–310 | Alpha/beta hydrolase family |
Abhydrolase_1 | PF00561.27 | 7.6e-08 | 52–137 | alpha/beta hydrolase fold |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: PE_PGRS37 (PE-PGRS family protein PE_PGRS37), high confidence from genomic context alone (score 767 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2126c PE_PGRS37 |
PE-PGRS family protein PE_PGRS37 | 767 | 767 ctx | cooccurence:767 |
Rv1243c PE_PGRS23 |
PE-PGRS family protein PE_PGRS23 | 733 | 733 ctx | cooccurence:733 |
Rv2353c PPE39 |
PPE family protein PPE39 | 592 | 592 ctx | cooccurence:591 |
Rv1703c |
methyltransferase | 570 | 571 ctx | cooccurence:563 |
Rv0124 PE_PGRS2 |
PE-PGRS family protein PE_PGRS2 | 567 | 567 ctx | cooccurence:567 |
Rv1918c PPE35 |
PPE family protein PPE35 | 557 | 557 ctx | cooccurence:557 |
Rv2627c hyp |
hypothetical protein | 569 | 554 | |
Rv3177 |
peroxidase | 553 | 553 ctx | neighborhood:540 |
Rv2853 PE_PGRS48 |
PE-PGRS family protein PE_PGRS48 | 552 | 552 ctx | cooccurence:552 |
Rv3825c pks2 |
phthioceranic/hydroxyphthioceranic acid synthase | 531 | 503 | experimental:441 |
Rv2933 ppsC |
phthiocerol synthesis polyketide synthase type I PpsC | 529 | 501 | experimental:441 |
Rv2940c mas |
multifunctional mycocerosic acid synthase | 529 | 501 | experimental:441 |
Rv1527c pks5 |
polyketide synthase | 528 | 501 | experimental:441 |
Rv2048c pks12 |
polyketide synthase | 526 | 499 | experimental:441 |
Rv1753c PPE24 |
PPE family protein PPE24 | 477 | 478 ctx | cooccurence:476 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): epoxide hydrolase MesT
- Pfam (hmmscan --cut_ga): Abhydrolase_6 PF12697.14 (E=2e-12), Abhydrolase_1 PF00561.27 (E=8e-08)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq YP_177938.1)
- Domains: Pfam-A via hmmscan --cut_ga — Abhydrolase_6 (PF12697.14), Abhydrolase_1 (PF00561.27)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0596 - Curated reference: UniProt Q6MX03 (TrEMBL, unreviewed; Predicted)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
30 functional partner(s); context anchor
PE_PGRS37 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv3176c|mesT MTHRASALISAQEWFSAGERVGYDAERPGINPRSPLRAFIRRAAGTGVTRTFLPGWPDGSYGWAKVEAFLSSRFHFPRIYLDYIGHGDSDKPRDYPYSTFERADLVEALWHAEGIAQTVVVAFDYSCIVSLELLARRIDRERAGNDQRTRITACLLANGGIFADGHTHAWYTTPLLTSPLGAAITPIGQRSWRMFAPFLRPVFSRGYPLSAAEMKELHDAISRRDGVRVLPATAGFVDEHREHAARWDLARIISALGDEVAFGVVGSAEDPFEGEQLRLARERLADSVEITELAGGHLTTAEQPDRLAEVIAALPERS