Rv1751 Resolved · high auto-curated
H37Rv Rv1751 · MTBC0 - ·
460 aa · 1979621–1981003 (+) ·
RefSeq NP_216267.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | oxidoreductase |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | Oxidoreductase. Pfam: FAD_binding_3 (PF01494.26), NAD_binding_8 (PF13450.13). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
O65936
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Probable oxidoreductase |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
C Energy production and conversionH Coenzyme transport and metabolism
|
|---|---|
| eggNOG description | COG0654 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases |
| Orthologous group | COG0654 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains) pseudogene candidate
| pN/pS | 0.723 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 4 synonymous, 7 missense, 1 nonsense, 0 frameshift |
| Disruption | 1 distinct premature-stop/frameshift site(s); most common in 1.66% of strains (2415) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
FAD_binding_3 | PF01494.26 | 1.5e-90 | 53–394 | FAD binding domain |
NAD_binding_8 | PF13450.13 | 7.2e-06 | 57–86 | NAD(P)-binding Rossmann-like domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: fadD1 (fatty-acid--CoA ligase FadD1), medium confidence from genomic context alone (score 571 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1752 hyp |
hypothetical protein | 972 | 787 ctx | neighborhood:759 textmining:878 |
Rv1814 erg3 |
membrane-bound C-5 sterol desaturase | 639 | 612 | coexpression:402 |
Rv1750c fadD1 |
fatty-acid--CoA ligase FadD1 | 581 | 571 ctx | neighborhood:559 |
Rv2402 |
trehalase | 544 | 544 ctx | neighborhood:544 |
Rv2401A |
membrane protein | 544 | 544 ctx | neighborhood:544 |
Rv1749c |
integral membrane protein | 437 | 438 ctx | neighborhood:412 |
Rv1745c idi |
isopentenyl-diphosphate delta-isomerase | 478 | 437 | coexpression:437 |
Rv1666c cyp139 |
cytochrome P450 Cyp139 | 439 | 418 | |
Rv3377c |
type B diterpene cyclase | 447 | 403 | coexpression:401 |
Rv0568 cyp135B1 |
cytochrome P450 Cyp135B1 | 420 | 399 | |
Rv3059 cyp136 |
cytochrome P450 Cyp136 | 407 | 385 | |
Rv1394c cyp132 |
cytochrome P450 Cyp132 | 405 | 383 | |
Rv0136 cyp138 |
cytochrome P450 Cyp138 | 404 | 382 | |
Rv2946c pks1 |
polyketide synthase | 441 | 270 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): oxidoreductase
- Pfam (hmmscan --cut_ga): FAD_binding_3 PF01494.26 (E=2e-90), NAD_binding_8 PF13450.13 (E=7e-06)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216267.1)
- Domains: Pfam-A via hmmscan --cut_ga — FAD_binding_3 (PF01494.26), NAD_binding_8 (PF13450.13)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0654 - Curated reference: UniProt O65936 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
14 functional partner(s); context anchor
fadD1 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv1751| MIATMPSMARRSRHDNKITTPAVDCLTIERLDSPASGAPQVTPYARALMGETTTCAIIGGGPAGMVLGLLLARAGVQVTLLEKHGDFLRDFRGDTVHPTTMRLLDELGLWERFAALPYSEVRTATLHSNGRAVTYIDFERLHQPYPYVAMVPQWDLLNLLAEAAQAEPSFTLRMKTEVTGLLREGGKVTGVRYQGAEGPGELRAELTVACDGRWSIARHEAGLKAREFPVNFDVWWFKLPREGDAEFSFLPRFSPGKGLGVIPREGYFQIAYLGPKGTDAQLRERGIEEFRRDVSELLPEATASVAALASMDEVKHLNVKVNRLRRWHIDGLLCIGDAAHAMSPVAGVGINLAVQDAVAAATILAEPLREHRVSSRHLAAVRRRRAFPTAVTQAVQRVLHRRLLGPLLQGRDPTPPAALLGLVERLPWLSAVPAYFVGVGVRPEHAPAFARRGPGNRKGP