Rv0797 Resolved · high auto-curated
H37Rv Rv0797 · MTBC0 - ·
364 aa · 890388–891482 (+) ·
RefSeq NP_215312.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | insertion sequence element IS1547 transposase |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | Insertion sequence element IS1547 transposase. Pfam: DEDD_Tnp_IS110 (PF01548.24), Transposase_20 (PF02371.23). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
O07182
TrEMBL · unreviewed
· Predicted
|
|---|---|
| UniProt name | Transposase for insertion sequence element IS1547 |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
L Replication, recombination and repair
|
|---|---|
| eggNOG description | Transposase IS116 IS110 IS902 |
| Orthologous group | COG3547 |
| KEGG orthology |
K07486
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 1.046 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 1 synonymous, 3 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
DEDD_Tnp_IS110 | PF01548.24 | 4.0e-24 | 4–155 | Transposase |
Transposase_20 | PF02371.23 | 2.6e-22 | 222–307 | Transposase IS116/IS110/IS902 family |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv0796 (Putative transposase for insertion sequence element IS6110; Involved in the transposition of the insertion sequence.), medium confidence from genomic context alone (score 630 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0796 |
Putative transposase for insertion sequence element IS6110; Involved in the transposition of the insertion sequence. | 631 | 630 ctx | neighborhood:608 |
Rv0795 |
Rv0795, (MTV042.05), len: 108 aa. Putative transposase for IS6110 (fragment), identical to Q50686 insertion element IS6110 (108 aa), FASTA s | 611 | 611 ctx | neighborhood:609 |
Rv0977 PE_PGRS16 |
PE-PGRS family protein PE_PGRS16 | 537 | 537 ctx | cooccurence:537 |
Rv1226c |
transmembrane protein | 449 | 449 ctx | cooccurence:441 |
Rv1870c hyp |
hypothetical protein | 441 | 442 ctx | cooccurence:425 |
Rv2098c PE_PGRS36 |
PE-PGRS family protein PE_PGRS36; Rv2098c, (MTCY49.38c), len: 434 aa. PE_PGRS36,Member of the Mycobacterium tuberculosis PE family, PGRS sub | 424 | 425 ctx | cooccurence:421 |
Rv0124 PE_PGRS2 |
PE-PGRS family protein PE_PGRS2 | 404 | 404 ctx | cooccurence:404 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): insertion sequence element IS1547 transposase
- Pfam (hmmscan --cut_ga): DEDD_Tnp_IS110 PF01548.24 (E=4e-24), Transposase_20 PF02371.23 (E=3e-22)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215312.1)
- Domains: Pfam-A via hmmscan --cut_ga — DEDD_Tnp_IS110 (PF01548.24), Transposase_20 (PF02371.23)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG3547 - Curated reference: UniProt O07182 (TrEMBL, unreviewed; Predicted)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
7 functional partner(s); context anchor
Rv0796 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv0797| MVVVGTDAHKYSHTFVATDEVGRQLGEKTVKATTAGHATAIMWAREQFGLELIWGIEDCRNMSARLERDLLAAGQQVVRVPTKLMAQTRKSARSRGKSDPIDALAVARAVMRETDLPLATHDETSRELKLLTDRRDVLVAQRTSAINRLRWLVHELDPERAPAARSLDAAKHQQALRTWLDTQPGLVAELARAELTDIIRLTGEINTLAQRISARVHQVAPALLEIPGCAELTAAKIVGEAAGVTRFKSEAAFACHAAVAPIPVWSGNTAGQMRLSRSGNRQLNAALHRIALTQIRMTDSRGQAYYQRLQDAGKTKRAALRCLKRRLARTVFQALRTVHQPSSEHTQPAAACHRSYCSRSCLSG