Rv1735c Family assigned · medium auto-curated
H37Rv Rv1735c · MTBC0 - ·
165 aa · 1961291–1961788 (-) ·
RefSeq NP_216251.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | membrane protein |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | Membrane protein. Pfam: SLAC1 (PF03595.23). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
P9WLS5
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Uncharacterized membrane protein Rv1735c |
UniProt still lists this protein as Uncharacterized membrane protein Rv1735c; the revised annotation above is ahead of the current UniProt record.
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
P Inorganic ion transport and metabolism
|
|---|---|
| eggNOG description | PFAM C4-dicarboxylate transporter malic acid transport protein |
| Orthologous group | COG1275 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.859 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 2 synonymous, 5 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
SLAC1 | PF03595.23 | 8.8e-10 | 1–118 | Voltage-dependent anion channel |
Functional interaction network (STRING v12, guilt-by-association)
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1997 ctpF |
cation transporter ATPase F | 858 | 800 | coexpression:800 |
Rv2624c |
universal stress protein | 740 | 740 | coexpression:734 |
Rv2028c |
universal stress protein | 737 | 737 | coexpression:731 |
Rv2625c rip3 |
zinc metalloprotease Rip3 | 841 | 731 | coexpression:731 textmining:436 |
Rv3128c |
Rv3128c, (MTCY164.38c), len: 337 aa. Conserved hypothetical protein, similar to other conserved hypothetical proteins. This ORF corresponds | 731 | 731 | coexpression:731 |
Rv2029c pfkB |
6-phosphofructokinase PfkB | 729 | 729 | coexpression:729 |
Rv1736c narX |
nitrate reductase-like protein NarX | 555 | 556 | |
Rv1734c hyp |
hypothetical protein | 537 | 537 ctx | neighborhood:416 |
Rv1164 narI |
nitrate reductase subunit gamma | 445 | 446 | |
Rv1737c narK2 |
nitrate/nitrite transporter | 528 | 429 | |
Rv2326c |
ABC transporter ATP-binding protein | 417 | 418 | coexpression:418 |
Rv3663c dppD |
dipeptide ABC transporter ATP-binding protein DppD | 415 | 416 | coexpression:416 |
Rv1161 narG |
nitrate reductase subunit alpha | 411 | 412 | |
Rv2032 acg |
NAD(P)H nitroreductase | 421 | 119 | |
Rv0570 nrdZ |
vitamin B12-dependent ribonucleoside-diphosphate reductase | 475 | 116 | textmining:431 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): membrane protein
- Pfam (hmmscan --cut_ga): SLAC1 PF03595.23 (E=9e-10)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216251.1)
- Domains: Pfam-A via hmmscan --cut_ga — SLAC1 (PF03595.23)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1275 - Curated reference: UniProt P9WLS5 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023, doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 — 31 functional partner(s)
- Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv1735c| MGATAITVLAGAHIVEMADAPMAIVTSGLVAGASVVFWAFGPWLIPPLVAASIWKHVVHRVPLRYEATLWSVVFPLGMYGVGAYRLGLAAHLPIVESIGEFEGWVALAVWTITFVAMLHHLAATIGRSGRSSHAIGAADDTHAIICRPPRSFDHQVRAFRRNQPM