Rv2327 Family assigned · medium auto-curated
H37Rv Rv2327 · MTBC0 mtbc0_002475 ·
163 aa · 2624737–2625228 (+) ·
RefSeq NP_216843.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | hypothetical protein |
|---|---|
| MTBC0 PGAP re-annotation | MarR family winged helix-turn-helix transcriptional regulator |
| Revised (this work) | MarR family winged helix-turn-helix transcriptional regulator. Pfam: MarR_2 (PF12802.14), MarR (PF01047.29), HTH_27 (PF13463.13). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P71885
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Conserved protein |
UniProt still lists this protein as Conserved protein; the revised annotation above is ahead of the current UniProt record.
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
K Transcription
|
|---|---|
| eggNOG description | MarR family |
| Orthologous group | COG1846 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.0 · strong purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 1 synonymous, 0 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
MarR_2 | PF12802.14 | 4.0e-11 | 43–100 | MarR family |
MarR | PF01047.29 | 1.4e-16 | 44–101 | MarR family |
HTH_27 | PF13463.13 | 6.9e-06 | 46–109 | Winged helix DNA-binding domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv2326c (ABC transporter ATP-binding protein), high confidence from genomic context alone (score 799 excluding text-mining). This association is the citable seed of a function hypothesis for this hypothetical protein.
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2326c |
ABC transporter ATP-binding protein | 799 | 799 ctx | neighborhood:789 |
Rv2325c hyp |
hypothetical protein | 797 | 798 ctx | neighborhood:789 |
Rv1510 hyp |
hypothetical protein | 514 | 514 ctx | cooccurence:479 |
Rv3630 |
integral membrane protein | 493 | 494 ctx | cooccurence:466 |
Rv1244 lpqZ |
lipoprotein LpqZ | 458 | 456 | |
Rv2328 PE23 |
PE family protein PE23 | 456 | 455 ctx | neighborhood:455 |
Rv2887 |
HTH-type transcriptional regulator | 428 | 408 ctx | cooccurence:404 |
Rv1634 |
multidrug-efflux transporter | 400 | 309 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: hypothetical protein
- MTBC0 PGAP product: MarR family winged helix-turn-helix transcriptional regulator
- Pfam (hmmscan --cut_ga): MarR_2 PF12802.14 (E=4e-11), MarR PF01047.29 (E=1e-16), HTH_27 PF13463.13 (E=7e-06)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216843.1)
- Domains: Pfam-A via hmmscan --cut_ga — MarR_2 (PF12802.14), MarR (PF01047.29), HTH_27 (PF13463.13)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1846 - Curated reference: UniProt P71885 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
8 functional partner(s); context anchor
Rv2326c - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_002475|Rv2327| MSPSPAAANRSEVGGPLPGLGADLLAVVARLNRLATQRIQMPLPAAQARLLATIEAQGEARIGDLAAVDHCSQPTMTTQVRRLEDAGLVTRTADPGDARAVRIRITPEGIRTLTAVRADRAAAIEPQLALLPPADRRVLADAVDVLRRLLDHAATTPGRATRQ