ncRv3461c MTB000156
ncRNA Putative small regulatory RNA
This is an RNA, not a coding gene: it belongs to a separate track and carries none of the protein evidence layers.
Location
3880231–3880294 (- strand), 64 nt, on H37Rv
(NC_000962.3).
Overlaps: Rv3461c.
Upstream neighbour Rv3460c
(161 bp).
Downstream neighbour Rv3462c
(138 bp).
Intra-MTBC variation
| Genomes surveyed | 145,209 |
| Variable sites | 22 |
| Variants reaching 0.1 % of genomes | 0 |
| Carriers of the most widespread variant | 21 |
Coverage control
Variant density inside this RNA: 34.4 sites/100 bp, versus 62.5 in the flanking ±500 bp (GC 71.9 %). The flanks act as an internal witness: if variants are called normally around the RNA but none becomes widespread inside it, the constraint is real rather than a sequencing gap.
Verdict: COUVERTURE OK -> invariance biologique plausible
Read with care: constraint is not function. This RNA mutates like its surroundings, but no variant spreads through the population — which is evidence of selection acting on it, not evidence of what it does. Secondary-structure pairing was tested across the RNA set and did not explain this constraint.
Predicted secondary structure
65.6 % of bases are paired in the minimum-free-energy fold (42 paired, 22 unpaired). Folded in isolation; the in vivo structure may differ (partners, co-transcriptional folding).
Legacy record
Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017)
Sources
- Descriptive record (locus, name, product, comment, coordinates): Mycobrowser v5, stable RNAs (Kapopoulou et al. 2011, doi:10.1016/j.tube.2010.09.006).
- Intra-MTBC variation and coverage control: computed for this atlas over 145,209 genomes.
- Secondary structure: ViennaRNA 2.7.2, MFE fold.