MTS2823 MTB000078

ncRNA Putative small regulatory RNA

This is an RNA, not a coding gene: it belongs to a separate track and carries none of the protein evidence layers.

Location

4100669–4100968 (+ strand), 300 nt, on H37Rv (NC_000962.3). Intergenic (no overlapping coding sequence). Upstream neighbour Rv3661 (159 bp). Downstream neighbour Rv3662c (297 bp).

Intra-MTBC variation

Genomes surveyed145,209
Variable sites325
Variants reaching 0.1 % of genomes 4
Carriers of the most widespread variant676

Coverage control

Variant density inside this RNA: 108.3 sites/100 bp, versus 71.4 in the flanking ±500 bp (GC 61.7 %). The flanks act as an internal witness: if variants are called normally around the RNA but none becomes widespread inside it, the constraint is real rather than a sequencing gap.

Verdict: n/a (a des variants communs)

Predicted secondary structure

62.7 % of bases are paired in the minimum-free-energy fold (188 paired, 112 unpaired). Folded in isolation; the in vivo structure may differ (partners, co-transcriptional folding).

Legacy record

MTS2823, putative small regulatory RNA (See Arnvig et al., 2011), 5'-end mapped by RLM-RACE, 3'-end not mapped, ~300 bp and ~250 bp bands detected by Northern blot.

Sources

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