mcr11 MTB000063

ncRNA Putative small regulatory RNA

This is an RNA, not a coding gene: it belongs to a separate track and carries none of the protein evidence layers.

Location

1413094–1413224 (- strand), 131 nt, on H37Rv (NC_000962.3). Intergenic (no overlapping coding sequence). Upstream neighbour Rv1264 (7 bp). Downstream neighbour Rv1265 (36 bp).

Intra-MTBC variation

Genomes surveyed145,209
Variable sites4
Variants reaching 0.1 % of genomes 0
Carriers of the most widespread variant6

Coverage control

Variant density inside this RNA: 95.4 sites/100 bp, versus 75.0 in the flanking ±500 bp (GC 66.4 %). The flanks act as an internal witness: if variants are called normally around the RNA but none becomes widespread inside it, the constraint is real rather than a sequencing gap.

Verdict: n/a (a des variants communs)

Read with care: constraint is not function. This RNA mutates like its surroundings, but no variant spreads through the population — which is evidence of selection acting on it, not evidence of what it does. Secondary-structure pairing was tested across the RNA set and did not explain this constraint.

Predicted secondary structure

61.1 % of bases are paired in the minimum-free-energy fold (80 paired, 51 unpaired). Folded in isolation; the in vivo structure may differ (partners, co-transcriptional folding).

Legacy record

mcr11, putative small regulatory RNA (See DiChiara et al., 2010). 5'-end mapped by RLM-RACE in M. tuberculosis H37Rv, 3'-end not mapped (See Arnvig et al., 2011).

Sources

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