ncRv3520 MTB000157

ncRNA Putative small regulatory RNA

This is an RNA, not a coding gene: it belongs to a separate track and carries none of the protein evidence layers.

Location

3956291–3956550 (+ strand), 260 nt, on H37Rv (NC_000962.3). Overlaps: Rv3520c. Upstream neighbour Rv3519 (31 bp). Downstream neighbour Rv3521 (971 bp).

Intra-MTBC variation

Genomes surveyed145,209
Variable sites187
Variants reaching 0.1 % of genomes 3
Carriers of the most widespread variant897

Coverage control

Variant density inside this RNA: 71.9 sites/100 bp, versus 72.6 in the flanking ±500 bp (GC 64.6 %). The flanks act as an internal witness: if variants are called normally around the RNA but none becomes widespread inside it, the constraint is real rather than a sequencing gap.

Verdict: n/a (a des variants communs)

Predicted secondary structure

72.3 % of bases are paired in the minimum-free-energy fold (188 paired, 72 unpaired). Folded in isolation; the in vivo structure may differ (partners, co-transcriptional folding).

Legacy record

Supported by sRNA-Seq in H37Rv (see DeJesus et al. 2017)

Sources

← all RNAs