mpr18 MTB000071

ncRNA Fragment of putative small regulatory RNA

This is an RNA, not a coding gene: it belongs to a separate track and carries none of the protein evidence layers.

Location

4093468–4093522 (+ strand), 55 nt, on H37Rv (NC_000962.3). Intergenic (no overlapping coding sequence). Upstream neighbour Rv3651 (590 bp). Downstream neighbour Rv3652 (110 bp).

Intra-MTBC variation

Genomes surveyed145,209
Variable sites49
Variants reaching 0.1 % of genomes 0
Carriers of the most widespread variant61

Coverage control

Variant density inside this RNA: 89.1 sites/100 bp, versus 79.0 in the flanking ±500 bp (GC 69.1 %). The flanks act as an internal witness: if variants are called normally around the RNA but none becomes widespread inside it, the constraint is real rather than a sequencing gap.

Verdict: COUVERTURE OK -> invariance biologique plausible

Read with care: constraint is not function. This RNA mutates like its surroundings, but no variant spreads through the population — which is evidence of selection acting on it, not evidence of what it does. Secondary-structure pairing was tested across the RNA set and did not explain this constraint.

Predicted secondary structure

61.8 % of bases are paired in the minimum-free-energy fold (34 paired, 21 unpaired). Folded in isolation; the in vivo structure may differ (partners, co-transcriptional folding).

Legacy record

mpr18, fragment of putative small regulatory RNA (See DiChiara et al., 2010), ends not mapped, 82 and 100 nt bands detected by Northern blot in M. bovis BCG Pasteur.

Sources

← all RNAs