mpr17 MTB000070

ncRNA Fragment of putative small regulatory RNA

This is an RNA, not a coding gene: it belongs to a separate track and carries none of the protein evidence layers.

Location

4040879–4040938 (+ strand), 60 nt, on H37Rv (NC_000962.3). Intergenic (no overlapping coding sequence). Upstream neighbour Rv3596c (175 bp). Downstream neighbour Rv3597c (43 bp).

Intra-MTBC variation

Genomes surveyed145,209
Variable sites40
Variants reaching 0.1 % of genomes 0
Carriers of the most widespread variant23

Coverage control

Variant density inside this RNA: 66.7 sites/100 bp, versus 53.2 in the flanking ±500 bp (GC 71.7 %). The flanks act as an internal witness: if variants are called normally around the RNA but none becomes widespread inside it, the constraint is real rather than a sequencing gap.

Verdict: COUVERTURE OK -> invariance biologique plausible

Read with care: constraint is not function. This RNA mutates like its surroundings, but no variant spreads through the population — which is evidence of selection acting on it, not evidence of what it does. Secondary-structure pairing was tested across the RNA set and did not explain this constraint.

Predicted secondary structure

53.3 % of bases are paired in the minimum-free-energy fold (32 paired, 28 unpaired). Folded in isolation; the in vivo structure may differ (partners, co-transcriptional folding).

Legacy record

mpr17, fragment of putative small regulatory RNA (See DiChiara et al., 2010), ends not mapped, 82-118 nt bands detected by Northern blot in M. bovis BCG Pasteur.

Sources

← all RNAs