mcr5 MTB000066

ncRNA Fragment of putative small regulatory RNA

This is an RNA, not a coding gene: it belongs to a separate track and carries none of the protein evidence layers.

Location

2437823–2437866 (- strand), 44 nt, on H37Rv (NC_000962.3). Overlaps: Rv2175c. Upstream neighbour Rv2174 (364 bp). Downstream neighbour Rv2176 (75 bp).

Intra-MTBC variation

Genomes surveyed145,209
Variable sites26
Variants reaching 0.1 % of genomes 1
Carriers of the most widespread variant16,503

Coverage control

Variant density inside this RNA: 59.1 sites/100 bp, versus 63.3 in the flanking ±500 bp (GC 65.9 %). The flanks act as an internal witness: if variants are called normally around the RNA but none becomes widespread inside it, the constraint is real rather than a sequencing gap.

Verdict: n/a (a des variants communs)

Predicted secondary structure

40.9 % of bases are paired in the minimum-free-energy fold (18 paired, 26 unpaired). Folded in isolation; the in vivo structure may differ (partners, co-transcriptional folding).

Legacy record

mcr5, fragment of putative small regulatory RNA (See DiChiara et al., 2010), cloned from M. bovis BCG Pasteur; ends not mapped, ~82 nt band detected by Northern blot.

Sources

← all RNAs